中药五加皮抗肿瘤作用体内外实验研究

目的：分离中药五加皮抗肿瘤活性物质，研究五加皮提取物在体内外的抗肿瘤细胞作用，为五加皮抗肿瘤新药开发、研制提供依据。经皮下注射肿瘤细胞建立荷瘤小鼠动物模型，通过灌胃给药检测五加皮提取物的体内抗肿瘤作用。方法：用^3H—TdR掺入法测定了肿瘤细胞增殖反应；用凝胶层析法初步分离、纯化了五加皮抗肿瘤成分。结果：五加皮提取物对多种组织来源的肿瘤细胞增殖有较强的抑制作用(P<0．01)，而且有较好的量效关系。五加皮提取物经口投入荷瘤小鼠后，实验组小鼠一般情况较对照组小鼠好，肿瘤生长较慢，生存期明显延长(P<0．01)。经凝胶层析分析，分离得到一种对肿瘤细胞增殖反应有抑制作用的蛋白质成分，分子量为64kda。结论：中药五加皮不仅在体外对多种肿瘤细胞增殖有抑制作用，在体内也有较好的抗肿瘤效果。其抗肿瘤活性与一种分子量为64kda的蛋白质成分有关，该成分的进一步分离、纯化和作用机理研究，对五加皮抗肿瘤作用的临床应用和新药开发提供科学依据。

Experimental Study on Anti-tumor Effects of Cortex Acanthopanacis Senticosus in Vivo and in Vitro

Objective:To provide a basis for development and preparation of the new anti-tumor agents from Cortex Acanthopanacis senticosus (CAS), through isolating the active substances from CAS and studying the anti-tumor effect of CAS extracts in vivo and in vitro. Methods:The effects of CAS extracts and its isolated ingredients on tumor cell proliferation in vitro was determined by 3H-TdR incorporation; the anti-tumor component of CAS was isolated and purified by chromatography; the tumor bearing mice model was established by injecting tumor cell subcutaneously, and the model was used to observe the anti-tumor effect of CAS extract administered through gastrogavage. Results:CAS extract showed obvious inhibition on trumor cell proliferation originated from multiple tissues (P<0.01) and displayed a better dose-effect relationship. After orally taken CAS extract, the general condition of mice in the experimental group were better than that in the untreated control group, revealing a slower growth and significantly prolonged survival period (P<0.01). A protein component, having inhibitory effect on tumor cell proliferation and the molecular weight was 64 kda, it was isolated by the thin layer gel chromatography. Conclusion:CAS has not only the in vitro inhibitory effect on cell proliferation of multiple kinds of tumor, but also a good anti-tumor effect in vivo. The anti-tumor activity of CAS is correlated with a protein component with the molecular weight of 64 kda. Further isolation, purification, study on mechanism will provide scientific evidence for clinical application and development of CAS in anti-tumor effect.