脂氧素A4抑制结缔组织生长因子诱导的肾小管上皮细胞表达整合素连接激酶

目的:结缔组织生长因子(connective tissue growth factor,CTGF)可诱导人肾小管上皮细胞HK-2表达整合素连接激酶(integrin-linked kinase,ILK),本研究观察脂氧素A4(lipoxinA4,LXA4)是否调节CTGF对合成ILK的作用,并探讨其作用机制。方法:对体外培养的人肾小管上皮细胞(HK-2细胞株),用不同浓度的LXA4预刺激,再加入CTGF共同孵育;或单用CTGF刺激HK-2细胞。应用RT-PCR和Westernblot方法测定ILKmRNA和蛋白表达,应用Westernblot法测定分裂原激活的蛋白激酶(p42/44mitogen-activated protein kinase,p42/44MAPK)(也称为ERK1/2)、磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3-K)。结果:CTGF刺激使HK-2细胞ILKmRNA表达和蛋白合成增加,磷酸化ERK1/2、磷酸化PI3-K的表达增加。LXA4呈剂量依赖性地抑制CTGF所致的上述变化。结论:LXA4可抑制CTGF引起的HK-2细胞表达ILK,其机制依赖于抑制...

Inhibition of CTGF-induced expression of integrin-linked kinase by lipoxin A4 in human renal tubular epithelial cells

Objective:Connective tissue growth factor(CTGF) can induce the expression of integrin-linked kinase(ILK)in human renal tubular epithelial cell line(HK-2).In this study,we investigated the modulatory effect of lipoxin A4(LXA4)on action of CTGFinduced ILK expression,and explored the mechanisms of the action of LXA4.Methods:Cultured HK-2 cells were stimulated with CTGF with or without pretreatment of LXA4.The expression of ILK mRNA was detected by RT-PCR.The expressions of ILK protein,p42/P44 mitogen-activated...