Pim-1在非小细胞肺癌中的表达及其与c-M yc的相关性

目的：研究原癌基因Pim‐1在非小细胞肺癌及癌旁正常组织中的表达情况及其与c‐M yc的相关性。方法收集该院胸外科手术切除的非小细胞肺癌患者的肺癌及癌旁正常肺组织标本各30例，统计临床病例资料及跟踪后期病理结果，利用RT‐PCR、qRT‐PCR及免疫组织化学方法检测肺癌及癌旁正常组织中 Pim‐1 mRNA、c‐Myc及 Pim‐1蛋白的表达，分析Pim‐1表达与临床病理特征及c‐Myc表达的相关性。结果在非小细胞肺癌中Pim‐1阳性率、mRNA及蛋白表达量明显高于癌旁组织， mRNA表达量分别为0．798±0．083和0．394±0．107（P＜0．01），蛋白阳性例数分别为18例及6例（P＝0．002）。Pim‐1蛋白的表达与非小细胞肺癌患者的年龄差距、性别差异、有无吸烟史、肿瘤病理类型及分化程度无关（P＞0．05），但与有无淋巴结转移及肿瘤的TNM分期有关（P＜0．05），有淋巴结转移及TNM分期升高，其表达量也随之上调。Pim‐1与c‐Myc蛋白表达呈正相关，相关系数（r）为0．433（P＝0．017）。结论 Pim‐1在非小细胞肺癌组织中呈高表达趋势，且随着存在淋巴结的转移及 TNM 分期的升高，其表达也随之增加。

The expression of Pim-1 in non-small cell lung cancer and its relationship with c-Myc

Objective To investigate the expression of Pim‐1 in non small cell lung cancer and adjacent normal tissues ,and study the relationship between c‐Myc and Pim‐1 in the corresponding tissue gene expression .Methods Totally 30 cases of non small cell lung cancer tissue and adjacent normal lung tissues were collected by surgical operation in department of thoracic surgery . Clinical data were statisticed and tracking late pathologic results ,using RT‐PCR ,qRT‐PCR and immunohistochemical method to de‐tect Pim‐1 mRNA ,c‐Myc and Pim‐1 protein expression in lung cancer and adjacent normal tissue ,and to analyze the relationship be‐tween the expression of Pim‐1 and c‐Myc .Results The positive rate of Pim‐1 mRNA and protein expression in non small cell lung cancer was obviously higher than that in adjacent normal tissue ,the mRNA expression levels were 0 .798 ± 0 .083 and 0 .394 ± 0 .107 (P<0 .01) ,the protein positive cases were 18 cases and 6 cases (P=0 .002) .The expression of Pim‐1 protein had no relationship on age ,gender ,smoking history ,pathological types and degree of differentiation of patients with non‐small cell lung cancer (P>0 .05) ,bue had related to ymph node metastasis and TNM stages of tumor (P<0 .05) ,with lymph node metastasis and TNM sta‐ges increases ,its expression quantity also rise .There was a positive correlation between Pim‐1 and c‐Myc protein expression ,corre‐lation coefficient (r) was 0 .433 (P=0 .017) .Conclusion High expression of Pim‐1 in non small cell lung cancer gene and is also increased with lymph node metastasis and TNM stages ,Pim‐1 and c‐Myc expression has positive correlation ,this could provide clues to the early diagnosis and prognosis evaluation of non small cell lung cancer ,and also provides a new train of thought and to find a new target for gene therapy of lung cancer .