反相高效液相色谱法测定犬脑组织和血浆中的异丙酚含量

目的建立测定犬脑组织中异丙酚含量的高效液相色谱方法。方法采用ZORBAXEclipseXDB-C18（4．6×150mm，5μm）为色谱柱，以甲醇-水（80：20，v／v）为流动相，流速1．0ml／min，检测波长为270nm。结果异丙酚的线性范围0．1～8μg／ml（r=0．9998），最低检测限和最低定量限为0.02μg／ml（S／N=3）和0．08μg／ml（S／N=10）。低、中、高浓度的平均回收率分别是89．5％、92．0％、96．16％，日内精密度和日间精密度分别为5．0％、4．2％、4．55％和6．74％、2．72％、5．07％。结论本方法快速、简单、准确，可用于测定犬脑组织和血浆中异丙酚的含量。

Determination of propofol in dog cerebral tissue and plasma by HPLC

Objective To develop an HPLC-UV method for determination of propofol in dog brain tissue. Methods Chromatography separation was performed on a ZORBAX Eclipse XDB-C18 （4. 6 × 150mm, 5μm） with the mobile phase consisted of methanol water （80/20, V/V） at room temperature. The flow rate was 1 ml/min and the monitor wave- length was 270nm. Results The linear range for propofol was 0. 1-8μg/ml with r=0. 9998. The LOD and LOQ were 0.02vg/ml（S/N= 3） and 0. 08μg/ml （S/N= 10）, respectively. The average recoveries of low, medium and high concentration were 89.5%, 92.0% and 96.16 %, respectively. The RSD% of intra-day （n= 5） and inter-day （n= 3） was below 7%. Conclusions The method was sensitive, accurate, precise and reliable for the determination of propofol in dog brain tissue.