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| 反相高效液相色谱法测定犬脑组织和血浆中的异丙酚含量 | |
| 引用本文： | 周枝凤,陈凌云,谢丰永,林春水,马安德.反相高效液相色谱法测定犬脑组织和血浆中的异丙酚含量[J].西部医学,2010,22(3):406-407,411. |
| 作者姓名： | 周枝凤 陈凌云 谢丰永 林春水 马安德 |
| 作者单位： | 1. 南方医科大学公共卫生与热带医学学院卫生检测中心,广东,广州,510515 2. 湖南省娄底市中心医院麻醉科,湖南,娄底,417000 3. 南方医科大学附属南方医院麻醉科,广东,广州,510515 |
| 摘 要： | 目的建立测定犬脑组织中异丙酚含量的高效液相色谱方法。方法采用ZORBAXEclipseXDB-C18（4．6×150mm，5μm）为色谱柱，以甲醇-水（80：20，v／v）为流动相，流速1．0ml／min，检测波长为270nm。结果异丙酚的线性范围0．1～8μg／ml（r=0．9998），最低检测限和最低定量限为0.02μg／ml（S／N=3）和0．08μg／ml（S／N=10）。低、中、高浓度的平均回收率分别是89．5％、92．0％、96．16％，日内精密度和日间精密度分别为5．0％、4．2％、4．55％和6．74％、2．72％、5．07％。结论本方法快速、简单、准确，可用于测定犬脑组织和血浆中异丙酚的含量。 |
| 关 键 词： | 异丙酚 高效液相色谱 脑组织 犬血浆 |
Determination of propofol in dog cerebral tissue and plasma by HPLC |
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| ZHOU Zhi-feng,CHEN Ling-yun,XIE Feng-yong,et al.Determination of propofol in dog cerebral tissue and plasma by HPLC[J].Medical Journal of West China,2010,22(3):406-407,411. | |
| Authors: | ZHOU Zhi-feng CHEN Ling-yun XIE Feng-yong |
| Institution: | ZHOU Zhi-feng1,CHEN Ling-yun1,XIE Feng-yong2,et al(1.Hygiene Detection Center,School of Public Health , Tropical Medicine,Southern Medical University,Guangzhou 510515,China,2.Department of Anesthesiology,Loudi Central Hospital,Loudi 417000,Hunan,3.Department of Anesthesiology,Nanfang Hospital,China) |
| Abstract: | Objective To develop an HPLC-UV method for determination of propofol in dog brain tissue. Methods Chromatography separation was performed on a ZORBAX Eclipse XDB-C18 （4. 6 × 150mm, 5μm） with the mobile phase consisted of methanol water （80/20, V/V） at room temperature. The flow rate was 1 ml/min and the monitor wave- length was 270nm. Results The linear range for propofol was 0. 1-8μg/ml with r=0. 9998. The LOD and LOQ were 0.02vg/ml（S/N= 3） and 0. 08μg/ml （S/N= 10）, respectively. The average recoveries of low, medium and high concentration were 89.5%, 92.0% and 96.16 %, respectively. The RSD% of intra-day （n= 5） and inter-day （n= 3） was below 7%. Conclusions The method was sensitive, accurate, precise and reliable for the determination of propofol in dog brain tissue. |
| Keywords: | Propofol HPLC Cerebral tissue Dog plasma |
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