| 摘 要： | Objective: To observe the influence of the "Warming yang and Nourishing qi" acupuncture treatment on the serum interleukin-12(IL-12) and interleukin-18(IL-18) expression levels in rats with experimental autoimmune myasthenia gravis(EAMG) and to analyze the mechanism of acupuncture in treatment of myasthenia gravis(MG).Methods: AchRα1 129-145 peptide fragments were used to immune the female Lewis rats to establish EAMG rat models.Thirty EAMG model rats were randomly divided into acupuncture treatment group, drug control group and model control group(n=10); the unmodeled 10 rats purchased at the same period were used as the blank control group.The rats in acupuncture treatment group were treated by "Warming yang and Nourishing qi" acupuncture treatment, 30 min, once a day, 7 d for a period, 1 d rest between two periods, and two periods of continuous treatment.The rats in drug control group were treated by the pyridostigmine lavaged treatment, 18.5 mg/(kg·d), continuous treatment for 15 d.The rats in the other two groups had no any special treatments.After treatment, the blood samples from the vein of tail root of the rats in 4 groups were collected, and the serum expression levels of IL-12 and IL-18 were detected with ELISA method.Results: After treatment, compared with the model control group, the serum IL-12 and IL-18 expression levels of the EAMG rats in the acupuncture treatment group and the drugcontrol group were significantly decreased(P0.05 or P0.01).Compared between the acupuncture treatment group and the drug control group, the serum IL-12 and IL-18 expression levels of the EAMG rats were not significantly different(P0.05).Conclusion: "Warming yang and Nourishing qi" acupuncture treatment can reduce the serum IL-12 and IL-18 expression levels of EAMG rats.For the treatment of MG, the effect of acupuncture was similar to pyridostigmine.
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