柱前衍生化RP-HPLC法测定肌肽的含量

目的建立简单、快速测定肌肽含量的方法。方法采用2,4-二硝基氯苯柱前衍生化RP-HPLC法。衍生化试剂:2,4-二硝基氯苯;色谱柱:DiamonsilTM C18柱(200 mm×4.6 mm,5μm);流动相:乙腈-0.15 mol.L-1乙酸钠缓冲溶液(体积比1∶5);流速:1.0 mL.min-1;检测波长:360 nm。结果肌肽质量浓度在2.0~20.0 mg.L-1(r=0.999 4)内与其衍生化物峰面积呈良好的线性关系,平均回收率为96.8%,RSD为0.8%(n=9)。结论2,4-二硝基氯苯柱前衍生化RP-HPLC法可用于测定肌肽含量。

Content determination of carnosion by pre-column derivatization with PR-HPLC

Objective To estabish a pre-column derivatization with RP-HPLC method for the determination of content of carnosion. Methods 2,4-Dinitrochlorobenzene was used as derivatization regent. The reversed phase HPLC system consisted of a DiamonsilTM C₁₈ column(250 mm×4.6 mm，5 μm) and a mixture of acetonitrile:Sodium acetate solution (V:V=1:5 )as mobile phase.The flow rate was 1.0 mL·min^–1.The UV detection wavelength was 360 nm．Results A linearity between major derivative peak area and tiopronin concentration was achieved in the range of 2.0 -20.0 mg·L^-1, with r = 0.999 4. The average recovery was 96.8 % and RSD was 0.8 %( n = 9).Conclusion The method can be used for determination of content of carnosion．