NM23H1和p53在肝细胞及癌旁组织的表达

目的 本实验采用免疫组织化学LSAB法，联合检测NM23H1和p53在肝细胞癌原发灶，肝内转移灶和癌旁肝组织中的表达。结果 在癌旁肝组织中，NM23H1表达最高，原发灶较其肝内转移灶表达高。提示NM23H1高表达的肝细胞癌患者术后复发率低，预后较好；p53在癌旁肝组织中无表达，而在原发灶及肝内转移灶中呈显著高表达。提示p53高表达的肝细胞癌患者术后复发率较高，预后较差。在肝原发灶及其肝内转移灶和癌旁肝组织中，NM23H1和p53的表达呈负相关；与患者年龄、性别、肿瘤体积和肝细胞癌组织学类型无相关性，提示NM23H1和p53基因及其蛋白产物在肝细胞癌发生演进过程中起着重要的调控作用。而NM23H1和p53基因及其蛋白产物间相互或协同作用，即p53基因突变或失活后：NM23H1基因随之失活，从而导致NM23H1基因对肝细胞癌癌细胞转移抑制负调控作用的表达，可能是肝细胞癌发生肝内转移的重要分子调控机制之一。

Expression of NM23H1 and p53 in human hepatocellular carcinoma and its surrounding liver tissues

AIM The expresslon of NM23H1 and p53 was studied in primary focus of human hepatoccllular carcinoma, its metastases and its surrounding liver tissues with immunobistochemical LSAB method. The results showed that the expression of NM23H1 in surrounding liver tissues was the highest, the expression of NM23H1 in primary focus of human hepatocellular carcinoma was higher than that in its metastases. These results suggest that the recurrence rate after operation is lower in the hepatocellular carcinoma patient with the high expression of NM23H1 and their prognosis is carcinoma, and its expression better. There was ntexpreession of p53 in surrounding liver tissues of humanhepatocellular was very prominent in primary focus and metasases of that. These results suggest that the recurrence rate after operation is higher in the ehpatocdllular carcinoma patients with high expression of p53 and their prognosis is worse. The expression of NM23H1 and p53was negative correlation in primary focus of human hepatocellular carcinoma, its metastases and surrounding liver tissues, and their expression was not related to age, sex, tumor's volume and histologic pattem of hument hepatocellular carcinoma. These results suggest that the gene of NM23H1 and p53 and their expression protein plays a important regulation role in the evolution process of human hepatocellular carcinoma. The action of NM23H1 and p53 gene and their expression protein is mutual or coordinate, namely NM23H1 gene devitalizes mmediately after p53 gene mutates or devtalizes and there by causing negative regulation's expression of NM23H1 gene to cartcinomatous cell's metastasis restraint of human hepatocellular carcinoma. This may be one of the most important molecular regulation mechanism of the human hepatocellular carcinoma metastasis within liver.