异常高氡温泉周围居民外周血中肺癌相关基因和miRNA的表达

目的 探讨四川省若尔盖县降扎乡异常高氡温泉对周围居民外周血中肺癌相关基因和miRNA表达的影响.方法 获取高氡温泉及对照地区居民外周血样,用实时RT-PCR检测肺癌相关基因( p53、k-ras)及血浆中相关miRNA(let-7a、miR-34a/b)的表达,用Western blot检测靶蛋白p53和k-ras的表达.结果 高氡温泉周围居民p53和k-ras基因mRNA表达分别是对照组的0.97和1.33倍,但差异无统计学意义(t=0.13、-1.12,P＞0.05)；靶蛋白p53和k-ras的表达与基因表达变化趋势一致,分别是对照组的0.7倍和1.23倍,(t=0.72、0.46,P＞0.05).高氡组let-7a与对照相比有下降趋势(t=1.63,P＞0.05).值得注意的是,与对照组相比,高氡组miR-34a和miR-34b明显高表达(t=-3.20、-3.32,P＜0.05).结论 通过检测p53和k-ras基因以及miRNA的变化,推测高氡温泉周围居民受到了辐射损伤.

Expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding hot springs with extremely high radon

Objective To investigate the expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding the extremely high radon hot springs in Ruoergai County, Sichuan Province. Methods Peripheral blood samples were collected from the local residents. Expressions of lung cancer related genes (p53, k-ras) and miRNA (let-7a, miR-34a/b) were detected by real-time PCR and the protein expressions of p53 and k-ras were detected by Western blot. Results The expressions of p53 and k-ras mRNA of the residents in high radon area were 0.97 and 1.33 times of the control respectively (t=0.13,-1.12,P>0.05), and the p53 and k-ras protein levels were 0.70 and 1.23 times of the control respectively (t=0.72,0.46,P>0.05). The let-7a of the residents in high radon area was lower (t=1.63,P>0.05) while the miR-34a and miR-34b were significantly higher than those of the controls (t=-3.20,-3.32,P<0.05). Conclusions Based on the expressions of p53 and k-ras gene and miRNA, it can be concluded that the residents surrounding the high radon hot springs received radiation damage.