| 簇分化抗原74基因转染对人脐静脉内皮细胞ECV304特性的影响 |
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| 引用本文： | 姜艳红.簇分化抗原74基因转染对人脐静脉内皮细胞ECV304特性的影响[J].华南国防医学杂志,2011,25(5):386-390. |
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| 作者姓名： | 姜艳红 |
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| 作者单位： | 河南省肿瘤医院检验科,河南郑州,450008 |
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| 摘 要： | 目的 探讨簇分化抗原74(cluster of differentiation 74,CD74)基因在人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)的转染条件和CD74基因转染对细胞特性及功能的影响,为深入研究基于调节CD74表达的生物治疗打下基础.方法 ①将...
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| 关 键 词： | 簇分化抗原74 人脐静脉内皮细胞 基因转染 人类白细胞抗原 |
Impact of CD74 Gene Transfection on Characteristics of Human Umbilical Vein Endothelial Cell ECV304 |
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| JIANG Yan-hong.Impact of CD74 Gene Transfection on Characteristics of Human Umbilical Vein Endothelial Cell ECV304[J].Military Medical Journal of South China,2011,25(5):386-390. |
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| Authors: | JIANG Yan-hong |
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| Institution: | JIANG Yan-hong.Clinical Laboratory,Henan Tumor Hospital,Zhengzhou Henan 450008,China |
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| Abstract: | Objective To explore the transfecting conditions of the cluster of differentiation(CD74) gene in human umbilical vein endothelial cells and impact of CD74 on cell characteristics and functions,and lay the basis for biotherapy based on CD74 expression modulation. Methods PCMV-CD74 enhanced green fluorescent protein(EGFP) plasmid was transformed into E.coli,extracted,enzyme-cut and sequenced.The EGFP fluorescent plasmid was transfected to ECV304 and the transfection efficiency was detected.The expressions of ... |
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| Keywords: | Cluster of differentiation 74 Human umbilical vein endothelial cells Gene transfection Human leukocyte antigen |
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