| 香橼药材高效液相指纹图谱研究 |
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| 引用本文： | 毛淑杰,朱景宁,顾雪竹,李先端,林淑芬. 香橼药材高效液相指纹图谱研究[J]. 中药材, 2007, 30(8): 932-936 |
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| 作者姓名： | 毛淑杰 朱景宁 顾雪竹 李先端 林淑芬 |
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| 作者单位： | 中国中医科学院中药研究所,北京,100700 |
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| 基金项目： | 国家科技部基础性工作专项资金资助项目 |
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| 摘 要： | 目的：采用高效液相色谱法，研究并建立香橼2个品种药材的指纹图谱。方法：采用HPLC方法，用Kromsil C^18（4．6mm×250mm，5pm）色谱柱，甲醇和冰醋酸-水（3．1：100，v／v）梯度洗脱；流速1.0ml／min；检测波长284啪。结果：运用梯度洗脱得到的色谱图各色谱峰分离较好，达到中药指纹图谱研究的技术要求。结论：采用HPLC指纹图谱分析，可对香橼2个品种药材的质量作出评价。
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| 关 键 词： | 香橼 指纹图谱 高效液相色谱法 |
| 文章编号： | 1001-4454（2007）08-0932-05 |
| 修稿时间： | 2007-01-29 |
The HPLC Fingerprint Analysis of Fructus Citri |
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| MAO Shu-jie,ZHU Jin-ning,GU Xue-zhu,LI Xian-duan,LIN Shu-fen. The HPLC Fingerprint Analysis of Fructus Citri[J]. Journal of Chinese medicinal materials, 2007, 30(8): 932-936 |
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| Authors: | MAO Shu-jie ZHU Jin-ning GU Xue-zhu LI Xian-duan LIN Shu-fen |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China |
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| Abstract: | OBJECTIVE: To study and establish the fingerprint of raw Fructus Citri by phased high performance liquid chromatography. METHOD: The chromatographic conditions were as follows: a Kromasil C18 column was used; the mobile phase was composed of methanol and acetic-water(3.1:100, v/v) with gradient elution; the flow rate was 1.0 ml/min and the UV absorbance detection was set at 284 nm. RESULT: Under the selected chromatographic conditions, good HPLC fingerprints of Fructus Citri were obtained. CONCLUSION: Quality of raw Fructus Citri can be controlled effectively by HPLC fingerprint. |
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| Keywords: | Fructus Citri Fingerprint HPLC |
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