视网膜色素上皮和视杆细胞外节膜表面受体和配体的定位和分布

目的：探讨甘露糖受体与配体在视网膜色素上皮（RPE）细胞表面及视杆细胞外节（ROS）膜的表面的存在和分布。 方法：将12只Wistar大鼠的视网膜组织分为实验组和对照组，采用胶体金吸附过氧化酶，再经亲和法与刀豆球蛋白A（ConA）结合标记ConA受体的方法进行研究。 结果：实验组RPE细胞微绒毛及RPE顶部的ROS膜表面均有胶体金标记物，呑入RPE细胞内的ROS表面无胶体金标记物。 结论：RPE细胞和ROS膜表面有甘露糖受体和配体存在。 （中华眼底病杂志，1995，11：244-246）

DIDTRIBUTION OF RECEPTORS ON RPE CELLS AND LIGANDS ON ROS SURFACE

PURPOSE:To define the distribution of mannose receptors and ligands on the mi-crovilla of RPE cell and surface of rod outer segment (ROS)METHODS:The retinal explants of12 wistar rats were used in this study for 2 groups:experiment and control group.The receptors on the RPE membrane and ligands on surface of ROS were analysed by the way of studying Con A re-ceptor in which G-HRP combined with Con A by affinity method.RESULTS:The result indicated that in experiment group,G-probe existed on both RPE cell microvilla and ROS surface,and no G-probe on the ROS was phagocytized into RPE cells. Control group had no G-probe CONCLU-SIONS:Mannose receptor and ligand did distribute on RPE cell membrane and ROS surface.