激光损伤视网膜后神经元凋亡及GFAP表达的研究

目的 观察兔视网膜激光损伤后神经元细胞有无凋亡改变及视网膜Ｍｕｌｌｅｒ细胞胶质纤维酸性蛋白（ＧＦＡＰ）的表达变化。方法 应用末脱氧核苷酸转移酶介导的ｄＵＴＰ－Ｘ切口末标记法（ＴＵＮＥＬ法）标记凋亡细胞。应用免疫组化染色显示视网膜Ｍｕｌｌｅｒ细胞ＧＦＡＰ表示。结果伤后６ｈ、１、３、７ｄ视网膜各层可见散在分布的ＴＵＮＥＬ阳性凋亡细胞，尤以外核层多见，伤后３ｄ，视网膜可见Ｍｕｌｌｅｒ细胞ＧＦＡＰ表达；伤

Study of the expression changes of glial fibrillary acid protein on laser-induced apoptosis of retinal neurons in rabbits

Objective To determine the mechanism of retinal neuron death and the expression changes of GFAP by retinal Muller cells after laser injury.Methods A YAG laser was used to produce retina lesion in rabbits.Terminal deoxy nucleotidyl-transferase mediated dUTP and nick labelling(TUNEL)staining was used to identify the apoptotic neurons.The expression of GFAP by retinal Muller cells was located by immunohistochemistry.Results TUNEL positive apoptotic neurons were located in all the layers of retina in the 6th h our and on the 1st,3rd,and 7th day after injury .Apoptotic cells reduced in number on the 14th day after injury.The TUNEL positive cells were more densely located in the outer nuclear layer.The Muller cells expresse of GFAP starting to increase at 3 days after injury,and increased co ntinuously later.Conclusion Apoptosis is an import ant mechanism of retinal neuron death i n laser-injured retina,and the increased expression of GFAP by Muller cells is probably related to repair of the injury.