多层螺旋CT观察矽肺动物模型疗效的可行性研究

目的 探讨采用多层螺旋CT（multi-slice spiral CT，MSCT）观察大鼠矽肺模型疗效的可行性。
方法 将60只无特定病原级SD大鼠随机分为对照组、模型组和治疗组3组，每组各20只，分别进行MSCT检查及病理检查。根据各个肺窗的小阴影密集度拟定大鼠矽肺模型MSCT分级，统计所有大鼠矽肺模型MSCT级别的例数。通过观察肺组织病理学改变，进行大鼠矽肺病理分级。不同组别大鼠矽肺模型MSCT分级、病理分级统计均采用非参数检验（Kruskal-Wallis检验）；大鼠矽肺模型MSCT分级与病理分级的一致性采用加权Kappa一致性检验。
结果 不同组别大鼠矽肺模型MSCT分级（H=26.27，P < 0.01）及病理分级（H=38.86，P < 0.01）的分布差异均有统计学意义。大鼠矽肺模型MSCT分级与病理分级的一致性检验的加权Kappa值=0.706（P < 0.05），提示各组大鼠的矽肺模型MSCT分级与病理分级具有较高的一致性。
结论 MSCT可观察大鼠矽肺模型的疾病状态发展，作为评估大鼠矽肺模型疗效的检查方法，具有一定的可行性。

Feasibility study of multi-slice spiral CT in observing the curative effect of silicosis animal model

Objective To investigate the feasibility of multi-slice spiral CT (MSCT)examination in animal silicosis model, the MSCT examination results were compared with the pathological examination result in rat silicosis model. Methods Sixty rats without specific pathogen grade were divided into control group, silicosis model group and hUC-MSCs treatment group. After 60 days, all rats were examined by MSCT and finally received pathological examination. The MSCT classification of rat silicosis model was made according to the small shadow density of each lung window. The pathological grades of the rat silicosis model were determined according to the percentage of the area of the inflammatory granuloma, silicotic nodule and silicotic fibrosis in the lung tissue. The MSCT classification and pathological grading in different groups of rats with silicosis were compared by nonparametric test (Kruskal-Wallis test); the consistency of MSCT classification and pathological grading in rat silicosis model was tested by weighted Kappa consistency test. Results Both MSCT classification (H=26.27, P < 0.01)and pathological grade (H=38.86, P < 0.01) showed that there were statistically significant differences among different groups. The consistency test (Kappa=0.706, P < 0.05) suggested that the MSCT classification and pathological grade in rat silicosis model were higher consistent. Conclusion MSCT examination, as a non-fatal examination, can observe the development of silicosis stage in rat silicosis model, and it can be used as an examination method to track the effect of stem cell treatment on rat silicosis model.