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| X线对舌鳞癌细胞系Tca8113端粒长度影响的动态观察 | |
| 引用本文： | 谢谦,黄洪章,魏菁,毛永林,胡晓文,余东升.X线对舌鳞癌细胞系Tca8113端粒长度影响的动态观察[J].中山大学学报(医学科学版),2004,25(5):454-457. |
| 作者姓名： | 谢谦 黄洪章 魏菁 毛永林 胡晓文 余东升 |
| 作者单位： | 1. 中山大学附属第二医院,口腔颌面外科,广东,广州,510120 2. 中山大学附属第二医院,林百欣医学研究中心,广东,广州,510120 3. 中山大学附属第二医院,肿瘤科,广东,广州,510120 |
| 摘 要： | 目的]探讨放射线对人舌鳞癌细胞系Tca8113端粒长度变化的影响.方法]采用流式原位杂交法(FLOW-FISH)检测Tca8113细胞的端粒相对长度(RTL).结果]与未照射细胞相比,经X线处理后,Tca8113细胞RTL值明显下降(P＜0.05);在照射后1 h至3 h间,不同照射剂量的细胞RTL值均有一定程度上升;5 h时,除2 Gy组外,其它各照射剂量组的RTL值均下降.结论]经X线照射后,Tca8113细胞端粒明显缩短.端粒缩短可能是放射损伤的机制之一. |
| 关 键 词： | 端粒 荧光原位杂交 流式细胞术 X线 |
| 文章编号： | 1672-3554(2004)05-0454-04 |
| 修稿时间： | 2004年3月15日 |
Dynamic Observation on Effect of X-ray on Telomere Length in Squamous Cell Carcinoma Tongue Tca8113 Cell Line |
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| XIE Qian ,HUANG Hong-zhang ,WEI Jing ,MAO Yong-lin ,HU Xiao-wen ,YU Dong-sheng.Dynamic Observation on Effect of X-ray on Telomere Length in Squamous Cell Carcinoma Tongue Tca8113 Cell Line[J].Journal of Sun Yatsen University(Medical Sciences),2004,25(5):454-457. | |
| Authors: | XIE Qian HUANG Hong-zhang WEI Jing MAO Yong-lin HU Xiao-wen YU Dong-sheng |
| Institution: | XIE Qian 1,HUANG Hong-zhang 1,WEI Jing 2,MAO Yong-lin 3,HU Xiao-wen 1,YU Dong-sheng 1 |
| Abstract: | To investigate the effects of radiation on telomere length dynamics in squamous cell carcinoma tongue Tca8113cell line.Telomere length was detected in cultural cells of Tca8113by FLOW-FI SH and the relative telomere length(RTL)was analyzed.RTL values of Tca8113cells incubate d for various periods after exposure to various doses of X-ray radiation were much lower than those of non-radiated cells(P<0.05).After incubation for 1h to 3h,RTL values of Tca8113cells tre ated with various doses of X-ray irra diation rose to a certain extent.RTL values of the cells incubated for 5h after radiation decrease d except the group of 2Gy.Conclusion]The obvious shortening telomeres are observed in Tca8113cells treated w ith X-ray radiation.Telomere shortening may be one of the causes of radiation dama ge. |
| Keywords: | telomeres fluorescence in situ hybridization flow cytometry X-ray |
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