PDGF-B链基因三链形成寡核苷酸对C6胶质瘤细胞增殖和细胞周期的影响

目的观察PDGF-B链基因三链形成寡核苷酸(triplex-forming oligonucleotide,TFO)对C6胶质瘤细胞增殖和细胞周期的影响.方法应用免疫荧光流式细胞技术观察PDGF-B链基因TFO对C6胶质瘤细胞PDGF-B、PCNA表达的影响.应用流式细胞技术观察PDGF-B链基因TFO对C6胶质瘤细胞细胞周期的影响.结果 PDGF-B链基因TFO对C6胶质瘤细胞PDGF-B链基因、PCNA的表达有明显抑制作用,而且抑制作用存在浓度依赖性.PDGF-B链基因TFO能使C6胶质瘤细胞S期的百分率明显降低,阻止细胞由静止期(G0-G1期)进入(S期).结论 PDGF-B链基因TFO能够抑制C6胶质瘤细胞PDGF-B链基因的表达,阻碍细胞进入S期,降低细胞增殖能力.

The effects of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C6 glioma cells

Objective To clarify effect of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C 6 glioma cells. Methods The expressions of PDGF-B chain and PCNA in C 6 glioma cells after adding a set of dosages of TFO were quantified by immunofluorescence flow cytometric analysis.Cell cycle of C 6 glioma cells after adding a set of dosages of TFO were detected by flow cytometric analysis. Results TFO can obviously inhibit the expressions of PDGF-B chain and PCNA in C 6 glioma cells in concentration-dependent fashion. TFO can obviously inhibit the transit of cells from the G 0 -G 1 to S phase in concentration-dependent fashion. Conclusion TFO can effectively suppress the PDGF-B chain expression and the cell proliferation C 6 glioma cells. TFO also can effectively induce the C 6 glioma cells arrest in the G 1 phase of the cell cycle.