细胞凋亡、增殖和Fas/FasL系统在大肠癌中的作用

目的 :探讨细胞凋亡、增殖及相关基因Fas、FasL在大肠癌发生发展中的作用。方法 :用免疫组化SP法检测 4 0例大肠腺癌、15例腺瘤及 15例正常组织Fas、FasL、PCNA蛋白的表达 ,并以TUNEL法对相应标本行凋亡细胞检测。结果 :凋亡指数随肿瘤分化程度的降低呈下降趋势 ,与淋巴结转移相关 (P <0 .0 5 )。增殖指数随肿瘤分化程度的降低呈上升趋势 ,但与淋巴结转移无相关性 (P >0 .0 5 )。在正常粘膜、大肠腺瘤、大肠腺癌中Fas表达逐渐降低 ,FasL表达逐渐增高 ,均有显著性差异 (P <0 .0 5 )。结论 :Fas、FasL异常表达促使肿瘤细胞逃避自身免疫攻击 ,细胞凋亡、增殖的平衡失调及Fas FasL系统异常表达在大肠癌发生发展中起重要作用。

Effect of Apoptosis,Proliferation and Fas/FasL System in Colorectal Carcinoma

Objective: To investigate the role of apoptosis,PCNA and related genes Fas,FasL in the progression of colorectal carcinoma. Methods: The expression of Fas, FasL, PCNA protein were detected by immunohistochemical method in 15 cases of colorectal adenomas,40 cases of colorectal carcinomas and 15 cases of normal mucous. The TdT medated dUTP biotin nick and labeling (TUNEL) positive were counted in all cases as apoptosis index. Results: AI was higher in colorectal carcinoma than normal tissues and lower than colorectal adenoma. It was well correlated with the pathological grading and lymph node metastasis(P<0.05). PI was increased in whole procession, but it was not correlated with clinical pathologic characteristics (P>0.05). Fas was declined and FasL was increased in whole procession (P<0.05). Conclusion: The abnormal expression of Fas,FasL in colorectal carcinoma suggest that it is a mechanism of immune escape. The imbalance of cell apoptosis and proliferation takes part in the progression of colorectal carcinoma.