绞股蓝总皂甙对小鼠四氯化碳肝损伤肝组织一氧化氮和谷胱甘肽的影响

目的观察绞股蓝总皂甙（Gypenosides,GPs）对肝损伤小鼠肝组织一氧化氮（NO）和谷胱甘肽（Glu-tathione,GSH）含量的影响。方法给四氯化碳（CCl4）诱导的三组慢性肝损害小鼠灌喂不同剂量的GPs（0、75、150mg／kg），观察和比较各组小鼠肝功能（血清ALT活性和白蛋白含量）的变化，并测定肝组织NO和GSH的含量。结果肝损伤对照组（即0mg／kgGPs组）NO（80．0±19．0μg／g湿肝）明显高于正常对照组（43．1±18．9μg／g湿肝）（P＜0．05）；GSH（0．48±0.25mg／g湿肝）较正常对照组（0．79±0.19mg／g湿肝）明显降低（P＜0．05）。用GPs75mg／kg和150mg／kg灌胃治疗肝损伤小鼠3周，均能降低肝组织NO含量和增加GSH含量，其中75mg／kg组肝组织NO为68．4±23．7μg湿肝，GSH为0．54±0.34μg／g湿肝；150mg／kg组肝组织NO为52．3±12.7μg／g湿肝，GSH为0．62±0.30mg／g湿肝。150mg／kg组与肝损伤对照组比较，肝组织NO和GSH均有显著性差异（P＜0．05）。同时，小鼠血清白蛋白含量及A／G比值均较肝损伤对照组升高，ALT活性降性，并与GPs剂量的增加相关。结论GPs在保护小鼠CCl4肝损伤的同时能降低肝组织NO含量和升高肝组织GSH水平。

Effect of gypenosides on nitric oxide and reduced glutathione in injured mouse liver

Objective To study the effeCt of ~nosides (GPs) nitric oxide (NO) and abced glutathione (GSH) in miedmouse livers. Me~ The Mouse models of chen tetra chloride -- induced chmnic hepatic in imp were employed in aams study andGPs was used to bet the hepatic damaged face for ~ weeks in ~ gnups with dOSages of 0 twkg (coup 11 ), 75 mg/kg (coupm ), or 150 ndkg (group fi), nsortively. aver No an(l GsH were ~ as wen as serum A-LT and albdrin. ~ No (80. ot 19. 0 aug) was increed and asH (0. 48 1 0. 25 maal) decreased in injured mo~ livers (group 11 ), compe with the normal coneals (coup l, NO 43. I t 18. 9 aug: GSH 0. 79 t 0. 19 mg/g) (P < 0.05 ). aver NO and GSH contents in coup mwere 68. 41 23. 7 aug and 0. 54t0. 34 aug (P > 0.05, vs group 11 ), while th~ in group able were 52. 31 12. 7 augand 0. 62 t 0. 30 aug respectivel}' (P < 0. 05, vs gi'Oup 11 ). rrhere was a lower level of ALT and a higher level of albumen inserum of injured face, with a positive GPs dose -- dependentcy. Concboon GPs bruces NO content and enhances GSH level in carbon ted'a chloride injured mo~ liver, while improving liver function.