天竺葵花抗氧化作用的研究

 目的：研究天竺葵花的抗氧化作用。方法：用化学发光法研究天竺葵花粗提液对超氧阴离子自由基（O〈sub〉2〈/sub〉^-）和羟在自由基（·OH）的清除作用。用分光光度法研究它对1，1一二苯基-2-苦肼基自由基（DPPH·）的清除作用。结果：天竺葵花具有很强的清除自由基活性。其粗提液清除O〈sub〉2〈/sub〉^-的IC〈sub〉50〈/sub〉分别为3.5μg·ml〈sup〉-1〈/sup〉（红花），3.0μg·ml〈sup〉-1〈/sup〉(粉花)，1.6μg·ml〈sup〉-1〈/sup〉(浅粉花)；清除·OH 的IC〈sub〉50〈/sub〉分别为5.7μg·ml〈sup〉-1〈/sup〉(红花)，4.2μg·ml〈sup〉-1〈/sup〉（粉花），3.8μg·ml〈sup〉-1〈/sup〉(浅粉花)；清除DPPH.的IC〈sub〉50〈/sub〉分别为12μg·ml〈sup〉-1〈/sup〉(红花)，8.3μg·ml〈sup〉-1〈/sup〉(粉花)，5.7μg·ml〈sup〉-1〈/sup〉(浅粉花)，均与市售茶多酚的清除能力相近（其IC〈sub〉50〈/sub〉。分别为：1.1，4.1，5.4μg·ml〈sup〉-1〈/sup〉）。结论：天竺葵花可能成为一种新的高效天然杭氧化剂。

Study on antioxidative effect of Pelargonium hortorum Bailey

OBJECTIVE: To study the antioxidative effects of Pelargonium hortorum Bailey flower water extracts. METHODS: Scavenging effects of Pelargonium hortorum Bailey flower extracts on superoxide radical (O 2)and hydroxyl radical (·OH) was studied by a chemiluminescence assay,and the scavenging effect of them on 1,1 diphenyl 2 picryl hydrazyl (DPPH·) was studied by spectrophotometry. RESULTS: The scavenging activity of Pelargonium hortorum Bailey flower extracts on free radical was strong.Its IC₅₀on O2was 3.5 μg·ml^-1(red),3.0 μg·ml^-1(pink),1.6 μg·ml^-1(lihgt pink),its IC50on ·OH was 5.7 μg·ml^-1(red),4.2 μg·ml^-1(pink),3.8 μg·ml^-1(light pink),its IC₅₀on DPPH· was 12 μg·ml^-1(red),8.3 μg·ml^-1(pink),5.7 μg·ml^-1(light pink),similar to that of tea polyphenol(IC₅₀=1.1,4.1,5.4 μg·ml^-1). CONCLUSION: The Pelargonium hortorum Bailey flower can be used as an antioxidant.