聚乳酸材料对成骨样细胞增殖和分化的影响

目的 研究聚乳酸对成骨样细胞增殖和分化的影响。方法 电镜观察未处理和经预处理的聚乳酸膜。分别将未处理（材料组）和经预处理（处理组）的聚乳酸薄膜与成骨样细胞体外共同培养，观察细胞形态学变化、MTT测定细胞的增殖以及测定碱性磷酸酶（ALP）活性。结果 ①电镜结果显示未处理膜与处理后的膜有细微差别。②材料组细胞与对照组相比出现明显的聚集样生长，细胞形态构成以多角形、星形比例为高，材料组MTT及ALP测定结果均低于对照组（P＜0．05）。预处理组与对照组细胞形态学的差异仅在实验开始3d可以观察到；而细胞的增殖及ALP活性与对照组的差异无统计学意义。结论 预处理后的聚乳酸薄膜对成骨样细胞增殖和分化并无抑制作用．在某种程度上．经处理后反而对其有轻微的促进作用。

Effect of Polylactic Acid on the Proliferation and Differentiation of Osteoblast-like Cells

Objective To assess the effects of polylactic acid(PLA) on the proliferation and differentiation of UMR-106 osteoblast-like cells.Methods The ultrastructures of the surface of untreated and pretreated PLA films were observed under electron microscope.UMR-106 osteoblast-like cells were cultured with PLA film which was untreated or treated by some special agents together,then the cells morphology was observed,and methyl thiazolyl tetrazolium(MTT) assay and alkaline phosphatase(ALP) analysis were performed to detect the proliferation and differentiation.Results Tiny distinction between the untreated film and pretreated film was found under electron microscope.The cells of material group were seen to have grown in clusters,compared with control.And the proportion of polygon and star cells was higher in the material group than in the control.The values measured by ALP and MTT in the material group were lower than control,yet the distinction was undulatory in different phase of test.The difference in cells morphology between the pretreatment group and control was observed only in the first three days.The values measured by ALP and MTT in the pretreatment group were almost consistent with those in control,and even slightly higher than control sometimes.Conclusion PLA film does not have permanent inhibitory effect on the proliferation and differentiation of osteoblast-like cells.On the contrary,the treated PLA film may accelerate the proliferation and differentiation of cells to some extent.So,PLA film may have the potential for use as a good tissue-engineering frame in the treatment of bone disorders.