E838对辐射诱导哺乳动物细胞恶性转化的影响

目的 研究抗放药物E838对辐射诱导哺乳动物细胞恶性转化的影响。方法 用不同剂量的^137Csγ射线照射培养的CHO和CHL细胞，比较在有或没有E838的情况下，其对细胞集落形成率以及转化率的影响。结果 在3Gy照射剂量下，CHO细胞加药1μg/ml处理组集落形成率显著高于不加药组（P＜0．05），而转化率加药组比不加药组低且有统计学意义（P＜0．01）。CHL细胞在此范围内变化亦有同样的趋势，但结果无统计学意义（P＞0．05）。结论 E838在浓度范围内对辐射诱导哺乳动物细胞转化有一定的抑制作用。

The effects of E838 on malignant transformation of mammalian cell induced by radiation

Objective　To study the effects of an anti-radiation medicine E838 on malignant transformation of mammalian cell induced by radiation. Methods　 CHO and CHL cells were cultured in the RPMI 1640 medium with or without E838 and were irradiated with different doses of 137Cs γ-rays.The total number of colonies and type Ⅰ,Ⅱ and Ⅲ malignant transformation foci were counted for each group three weeks after irradiation. Results　 Statistic analysis indicated that the colony-forming rate of CHO cells in the groups cultured with E838(1 μg/ml) was significantly higher than that of groups without E838 after irradiation with 3 Gy 137Cs γ-rays(P＜0.05).The transformation rate of the group with E838 was lower than that of the group without(P＜0.01).There was a similar tendency in CHL cells at the same irradiation dose,without statistical significance. Conclusion　In the proper concentration range E838 shows certain inhibitory effects on malignant transformation of mammalian cells induced by radiation.