肾移植术后受者人巨细胞病毒巢式PCR检测

目的探讨巢式PCR检测肾移植术后受者人巨细胞病毒（HCMV）DNA的方法，并与ELISA法进行比较。方法针对HCMVAD169株IEA基因设计两对巢式PCR引物，对50例肾移植术后受体血、尿标本进行HCMVDNA检测。分离血清作ELISA检测IgG、IgM。结果血液巢式PCR阳性率56％，尿液阳性率46％；ELISA法阳性率：IgG82％，IgM28％，IgG＋IgM28％。两种方法的阳性率进行统计学分析，P〈0．05，存在统计学差异。结论巢式PCR是一种敏感、快速诊断HCMV感染的方法，灵敏度及特异性均高于传统ELISA法，并能弥补ELISA的假阴性。不仅为临床HCMV疾病的诊断与治疗，同时也为选择普遍性预防抑或症状发生前的预先治疗提供可靠的实验室依据。

Detection of Human Cytomegalovirus In Renal Transplant Recipients

Objective To compare nest polymerase chain reaction(Nest PCR) with enzyme-linked immunoabsordent assay(ELISA) in detecting human cytomegalovirus(HCMV) DNA in renal transplant recipients.Methods two pairs of Nest PCR primers were designed according to the sequence of IEA gene of HCMV AD 169 strain.The blood and urine samples from 50 recipients were detected with Nest PCR.And the levels of IgG and IgM in the same recipients were detected with ELISA.Results With Nest PCR,the positive rate of HCMV in blood and urine samples were 56% and 46% respectively.With ELISA,the positive rate of IgG,IgM and IgG IgM were 82%,28%,28% respectively.The difference between the two methods was significant(P<0.05).Conclusion Nest PCR is a sensitive and rapid method to detect HCMV infection,and its sensitivity and specificity were higher than ELISA.This conclusion provides experimental evidences for the diagnosis,prevention and therapy of HCMV infection.