不同产地蒲公英药材中总黄酮和咖啡酸含量分析与评价△

目的：建立不同产地蒲公英药材综合质量评价方法。方法：采用紫外一可见分光光度法测定蒲公英总黄酮含量，采用HPLC测定其咖啡酸的含量。结果：不同产地蒲公英总黄酮和咖啡酸的含量均差异较大，总黄酮含量在28．63—51．79mg·g^-1，咖啡酸含量在0．0076％～0．0424％。结论：以总黄酮和咖啡酸作为质量评价指标，建立的方法简单、快捷、准确，结果表明山东临沂蒲公英的质量较好。

Determination of Total Flavonoid and Caffeic Acid in Taraxacum officinale from Different Habitats

Objective： To establish a comprehensive quality evaluation method for Taraxacum officinale from different habitats. Methods： Using ultraviolet-visible spetrophotometry determine total flavonoid and using HPLC determine caffeic acid content. Results： The total flavonoid content is 28.63-51.79 mg·g-1 and the caffeic acid content is 0. 007 6% 4）. 042 4%, which are remarkably different in different habitats. Conclusion： Shandong linyi medicinal materials quality is better according to total flavonoid and caffeic acid content. The method is convenient, rapid and accurate, which will be helpful for quality assessment of Taraxacum officinale.