Formation of bone by isolated,cultured osteoblasts in millipore diffusion chambers |
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Authors: | D J Simmons G N Kent R L Jilka D M Scott M Fallon David V Cohn |
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Institution: | (1) Division of Orthopedic Surgery, Washington University, St. Louis, Missouri;(2) Research Laboratories, V.A. Medical Center, Kansas City, Missouri;(3) University of Kansas Medical Center, Kansas City, Missouri;(4) Department of Pathology, Jewish Hospital of St. Louis, St. Louis, Missouri, USA |
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Abstract: | Summary Osteoblast-like and osteoclast-like cells freed from neonatal calvaria by sequential enzymatic digestion after 6–7 days in
culture were placed in diffusion chambers and implanted in the peritoneal cavities of CD-1 mice. About half of the chambers
also contained a dead calvarium to test for the need of an “inducer.” After 20 days, 11 of 18 chambers containing the osteoblast-like
cells formed large foci of mineralized bone that corresponded to alkaline phosphatase activity throughout the chambers. Moreover,
only type I (i.e., bone) collagen was formed. Occasional deposits of bone were found in only 3 of 22 chambers containing the
osteoclast-like cells. The presence of dead bone did not affect any of the results. These data confirm the osteoblast-like
nature of the isolated cell populations and demonstrate that these cells retain their differentiated function in culture. |
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Keywords: | Bone formation Osteoblasts Osteoclasts Bone Induction Alkaline Phosphatase Cell Culture |
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