腹腔感染脓毒症小鼠外周血淋巴细胞CD30的动态表达

目的:探讨腹腔感染脓毒症的病理过程中Th2细胞的分化情况。方法:盲肠结扎穿孔(CLP)复制腹腔感染脓毒症小鼠模型,在不同时点取外周全血进行三色荧光标记,以流式细胞术对CD4⁺细胞表面CD30分子的表达情况进行分析。结果:不同时点的CLP组其CD30分子的表达有所不同,以术后38h为最高,随后呈现总体下降趋势。结论:在脓毒症腹腔感染小鼠的动物模型中,Th2细胞的分化情况随病程的发展有所不同,可能和疾病的严重程度及预后相关。

Dynamic expression of CD30 on peripheral blood lymphocytes in mice after a cecal ligation and puncture

AIM: To investigate the details of Th2 cell differentiation in septic mice. METHODS: Experimental septic mice were induced by cecal ligation and puncture (CLP). The exression of CD30 on CD4 +T cells at different time after CLP were estimated by flow cytometry following three-color immunofluorescent staining.RESULTS: CD30 expression on CD4 +T cell was different at each time point. The highest expression was showed at 38 h after CLP and declined later, which matched the changes in mortality of the animals. CONCLUSION: During sepsis, differentiation of Th2 cell changed with the development of sepsis and might be associated with the severity of the disease.