神经生长因子调控烧伤创面的实验研究

目的 研究神经生长因子（ＮＧＦ）在烧伤创面愈合的作用，探索烧伤创面愈合机制。方法２０ｋｇ小白家猪６只为实验对象。用控温控压电烫仪在每只猪背部制成２４个直径为２．５ｃｍ的圆形深Ⅱ度烧伤创面。分为四组，分别为创面局部应用１、２．５和５μｇ／ｍｌ的ＮＧＦ实验组和不含ＮＧＦ的生理盐水对照组。在用药后３、５和９天分别取创面组织进行表皮生长因子（ＥＧＦ）受体，ＥＧＦ，ＮＧＦ受体，ＮＧＦ，ＣＤ６８，ＣＤ３免疫组

MODULATION OF NERVE GROWTH FACTOR ON WOUND HEALING OF BURN

OBJECTIVE: To investigate the effect of nerve growth factor(NGF) on the burn wound healing and to study the mechanism of burn wound healing. METHODS: Six domestic pigs weighting around 20 kg were used as experimental animals. Twenty-four burn wound, each 2.5 cm in diameter, were induced on every pigs by scalding. Three different concentrations of NGF, 1 microgram/ml, 2.5 micrograms/ml, 5 micrograms/ml were topically applied after thermal injury, and saline solution used as control group. Biopsy specimens were taken at 3, 5 and 9 days following treatment and immunohistochemistry method was used to detect the epidermal growth factor(EGF), EGF receptor (EGF-R), NGF, NGF receptor (NGF-R), NGF, NGF-R, CD68 and CD3. RESULTS: The expression of EGF, EGF-R, NGF, NGF-R CD68 and CD3 were observed in the experimental group, especially at 5 and 9 days, no expression of those six items in the control group. CONCLUSION: NGF can not only act directly on burn wound, but also modulate other growth factors on the burn wound to accelerate the healing of burn wound.