Effects of benzo[a]pyrene exposure on oxidative stress and apoptosis of gill cells of Chlamys farreri in vitro |
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| Affiliation: | 1. Departamento de Biología, Instituto Nacional de Investigaciones Nucleares (ININ), Carretera México-Toluca, S/N, la Marquesa, Ocoyoacac CP. 52750, México;2. Facultad de Ciencias, Universidad Autónoma del Estado de México, Toluca, Edo Mex., México;1. Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, India;2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India;3. Developmental Toxicology Laboratory, Systems Toxicology & Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow 226001, India;4. Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, India;5. Pathology Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, India;1. Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California Davis, Davis, CA, USA;2. Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA;3. Mechanical and Aerospace Engineering, University of California, Davis, CA 95616, USA;4. Air Quality Research Center, University of California, Davis, Davis, CA, USA;5. Center for Health and the Environment, University of California, Davis, Davis, CA, USA;6. West Coast Metabolomics Center, Genome Center, University of California Davis, Davis, CA, USA;1. Department of Pharmacology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey;2. Department of Medical Biology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey;3. Department of Anatomy, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey;4. Department of Pathology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey |
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| Abstract: | As a common pollutant in marine environment, benzo[a]pyrene (B[a]P) has high toxicity to economic shellfish. In order to explore the mechanism of oxidative stress and apoptosis, the effects of 0, 2, 4, 8 μg/mL B[a]P on gill cells of C. farreri at 12 and 24 h were studied. The results showed that B[a]P decreased the activity of gill cells, increased the content of reactive oxygen species (ROS) and the expression of antioxidant defense genes. Besides, B[a]P could induce oxidative damage to nucleus and mitochondria. The gene expression and enzyme activity of apoptosis pathway related factors were changed. In conclusion, these results showed that B[a]P could cause oxidative stress and oxidative damage in gill cells of C. farreri, and mediate gill cell apoptosis through mitochondrial pathway and death receptor pathway. This article provides a theoretical basis for clarifying the molecular mechanism of PAHs-included oxidative stress and apoptosis in bivalves. |
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| Keywords: | Gill cell Oxidative stress Apoptosis |
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