The antibacterial activity of photodynamic agents against multidrug resistant bacteria causing wound infection |
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| Affiliation: | 1. Faculty of Health Sciences, Izmir Katip Çelebi University, Izmir Kâtip Çelebi Üniversitesi Balatçık Kampüsü, Çiğli, İzmir 35620, Turkey;2. Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Ege University, Turkey;3. Faculty of Engineering, Department of Biomedical Engineering, İzmir Katip Çelebi University, Turkey;4. Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Ege University, Turkey;6. Faculty of Medicine, Department of Microbiology, İzmir Katip Çelebi University, Turkey;2. The Department of Tumor Surgery, Lanzhou University Second Hospital, Lanzhou 730030, China;1. Department of Dermatology, Tongji Medical College, Wuhan No. 1 Hospital, Huazhong University of Science and Technology, Wuhan, China;2. Hubei Key Laboratory of Infectious and Immune Skin Diseases, Tongji Medical College, Wuhan No. 1 Hospital, Huazhong University of Science and Technology, Wuhan, China;1. Dermatology Department, Affiliated Dongguan Hospital, Southern Medical University, Dongguan, Guangdong, PR China;2. Dermatology Department, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, PR China;1. Department of Prosthetic Dental Sciences, Prince Sattam Bin Abdulaziz University, Al Kharj 11942, Saudi Arabia;2. Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University, P. O. Box 60169, Riyadh 11545, Saudi Arabia;3. Department Of Clinical Dental Sciences, College of Dentistry, Princess Nourah Bint Abdulrahman University, Saudi Arabia;1. Department of Chemistry, KG Reddy College of Engineering and Technology, Moinabad, Hyderabad, Telangana 500075, India;2. School of Mechanical Engineering, Beijing Institute of Technology, Beijing 100081, China;3. Department of Chemistry, College of Engineering Guindy, Anna University, Chennai, Tamil Nadu 600 0025, India;4. Department of Physics, St. Joseph''s College of Engineering, Chennai, Tamil Nadu, India;5. Department of Chemistry, C. Kandaswami Naidu College for Women, Cuddalore, Tamilnadu, India;6. Department of Biotechnology, Saveetha School of Engineering, SIMATS, Thandalam, Chennai, Tamilnadu 602105, India |
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| Abstract: | Antimicrobial photodynamic inactivation (aPDI) of multidrug-resistant (MDR) wound pathogens was evaluated with cationic porphyrin derivatives (CPDs). MDR bacterial strains including Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, and Klebsiella pneumoniae were used. The CPDs named PM, PE, PN, and PL were synthesized as a photosensitizer (PS). A diode laser with a wavelength of 655 nm was used as a light source. aPDI of the combinations formed with different energy densities (50, 100, and 150 J/cm²) and PS concentrations (ranging from 3.125 to 600 µM) were evaluated on each bacterial strain. Dark toxicity, cytotoxicity, and phototoxicity were determined on fibroblast cells. In the aPDI groups, survival reductions of up to 5.80 log₁₀ for E. coli, 5.90 log₁₀ for P. aeruginosa, 6.11 log₁₀ for K. pneumoniae, and 6.78 log₁₀ for A. baumannii were obtained. The cytotoxic effect of PL and PM on fibroblast cells was very limited. PN was the type of CPD with the highest dark toxicity on fibroblast cells. In terms of providing broad-spectrum aPDI without or with very limited cytotoxic effect, the best result was observed in aPDI application with PL. The other CPDs need some modifications to show bacterial selectivity for use at 50 µM and above. |
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| Keywords: | aPDI" },{" #name" :" keyword" ," $" :{" id" :" pc_4xz2tO2D07" }," $$" :[{" #name" :" text" ," _" :" Antimicr obial photodynamic inactivation CPDs" },{" #name" :" keyword" ," $" :{" id" :" pc_0lhX076lro" }," $$" :[{" #name" :" text" ," _" :" Cationic porphyrin derivatives PS" },{" #name" :" keyword" ," $" :{" id" :" pc_GgcEietv7C" }," $$" :[{" #name" :" text" ," _" :" photosensitizer MRSA" },{" #name" :" keyword" ," $" :{" id" :" pc_twfGGqU2vr" }," $$" :[{" #name" :" text" ," _" :" Methicillin -resistant Staphylococcus aureus DMSO" },{" #name" :" keyword" ," $" :{" id" :" pc_gWWjQM6Oke" }," $$" :[{" #name" :" text" ," _" :" Dimethyl sulfoxide MIC" },{" #name" :" keyword" ," $" :{" id" :" pc_glDiktdn5J" }," $$" :[{" #name" :" text" ," _" :" Minimal inhibitory concentrations |
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