In vitro antibacterial activity of Western Australian honeys,and manuka honey,against bacteria implicated in impetigo |
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| Institution: | 1. School of Biomedical Sciences, The University of Western Australia, Crawley, 6009, Australia;2. Cooperative Research Centre for Honey Bee Products Limited (CRC HBP), 128 Yanchep Beach Road, Yanchep, 6035, Australia;3. Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Nedlands, 6009, Australia;4. School of Medicine, The University of Western Australia, Crawley, 6009, Australia;5. Department of Infectious Diseases, Perth Children''s Hospital, Nedlands, 6009, Australia;1. The LoveYourBrain Foundation Windsor, Vermont, USA;2. Department of Psychiatry, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA;3. Department of Neurology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA;4. Department of Biomedical Data Science, Geisel School of Medicine, Lebanon, NH, USA;1. The Second School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China;2. Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming, 650500, Yunnan, China;3. Department of Rehabilitation Medicine, Affiliated Haikou Hospital of Central South University Xiangya School of Medicine, Haikou, 570208, Hainan, China;1. Department of Korean Medicine Cardiology and Neurology, Graduate School, Kyung Hee University, Seoul, Republic of Korea;2. Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea;1. Southern Cross University, Lismore NSW, Australia;2. Southern Cross University, Gold Coast, Queensland, Australia;3. Naturopath, Botanic Mind and Body, Australia;4. Naturopath, Dubbo Health Foods, Australia;1. South Texas Veteran Health Care System, Audie L. Murphy Veteran Hospital San Antonio, TX, USA;2. National Center for Homelessness Among Veterans, USA;3. Department of Psychiatry and Behavioral Sciences, Duke University School of Medicine, Durham, NC, USA;4. Durham Veterans Affairs Health Care System, Durham, NC, USA;5. Mid-Atlantic Mental Illness Research, Education, And Clinical Center (MIRECC), Durham, NC, USA;6. Department of Psychology, University of Toledo, Toledo, OH, USA;7. VA Salt Lake City Health Care System and IDEAS Center of Innovation, Salt Lake City UT, USA;8. University of Utah School of Medicine, Department of Medicine, Salt Lake City UT, USA;9. Durham HSRD Center (ADAPT), USA |
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| Abstract: | Impetigo is a contagious skin disease caused by Staphylococcus aureus and Streptococcus pyogenes. Without treatment, impetigo may be recurrent, develop into severe disease, or have serious, life-threatening sequelae. Standard treatment consists of topical or systemic antibiotic therapy (depending on severity), however, due to antibiotic resistance some therapies are increasingly ineffective. In this study we evaluated the potential for honey as an alternative treatment for impetigo. A broth microdilution assay in 96-well microtitre trays was used to determine the minimum inhibitory concentrations (MICs) of six monofloral honeys (jarrah, marri, red bell, banksia, wandoo, and manuka), a multifloral honey and artificial honey against S. aureus (n = 10), S. pyogenes (n = 10), and coagulase-negative staphylococci (CoNS) (n = 10). The optical density (OD) of all microtitre tray wells was also determined before and after assay incubation to analyse whether sub-MIC growth inhibition occurred. Jarrah, marri, red bell, banksia, and manuka honeys were highly effective at inhibiting S. aureus and CoNS, with MIC50 values ranging from 4 to 8% w/v honey. S. pyogenes was also inhibited by these same honeys, albeit at higher concentrations (8–29% w/v). Wandoo and multifloral honeys had the least antibacterial activity with MICs of >30% (w/v) for all isolates. However, OD data indicated that sub-MIC concentrations of honey were still partially restricting bacterial growth. Our pre-clinical data indicate that honey may be a potential therapeutic agent for the routine treatment of mild impetigo, and we suggest that clinical trials would be appropriate to further investigate this. |
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| Keywords: | Skin infection Natural product Apitherapy |
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