The impact of benzo[a]pyrene on murine allergic airway inflammation via epigenetic remodeling |
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| Affiliation: | 1. The Department of Anesthesiology, The Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen, China;2. Department of Anesthesiology, Peking University Shenzhen Hospital, Shenzhen, China;1. Division of Pharmacology & Toxicology, Defence Research Development & Establishment, Defence Research Development Organization, Gwalior 474002, India;2. Biotechnology Division, Defence Research Development & Establishment, Defence Research Development Organization, Gwalior 474002, India;1. School of Anesthesiology, Weifang Medical University, Weifang, China;2. Department of Anesthesiology, Peking University Shenzhen Hospital, Shenzhen, China;1. Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China;2. Department of Environmental Health, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Simultaneous exposure to both BaP and house dust mites (HDM) has been shown to exacerbate pulmonary inflammation and hyperresponsiveness in a murine asthma model. The mechanistic insight into epigenetic inheritance for this effect, however, remains to be clarified. As such, in this study, we explore the molecular basis for the enhancement of asthma. Female BAL/C mice were intranasally administered HDM (25 µg in 25 μL saline) and/or BaP (10 μg/kg) every other day for 9 weeks. RNA sequencing and DNA methylation assessment were used to explore the underlying mechanism. Following simultaneous exposure to HDM and BaP, mice exhibited pulmonary inflammation and the transcript level of IL4i1b, muc4 and IL22ra2 that were associated with altered DNA methylation, suggesting that there may be an epigenetic basis for BaP-induced asthma exacerbation. Our data suggest that DNA methylation is a major epigenetic modification that accompanies airway remodeling associated with changes in the allergic mice. |
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| Keywords: | Allergic airway inflammation Epigenetic remodeling |
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