| 自体骨髓间充质干细胞心肌移植后的分化及存活 |
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| 引用本文： | 蔡红雁,聂俊,陈丽星,赵玲,郭涛,肖践明.自体骨髓间充质干细胞心肌移植后的分化及存活[J].中国组织工程研究与临床康复,2010,14(6). |
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| 作者姓名： | 蔡红雁 聂俊 陈丽星 赵玲 郭涛 肖践明 |
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| 作者单位： | 昆明医学院第一附属医院心内科,云南省昆明市,650031 |
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| 基金项目： | 云南省教育厅基金资助项目 |
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| 摘 要： | 背景:骨髓间充质干细胞移植入急性心肌梗死组织后是否能迁移、分化及存活,目前尚不清楚.目的:观察兔骨髓间充质干细胞移植入急性心肌梗死组织后,在体内的迁移、分化及其存活.方法:分离培养兔骨髓间充质干细胞,体外DAPI标记细胞.结扎兔左前降支制作急性心肌梗死模型,造模成功后30只新西兰大耳兔抽签法分为骨髓间充质干细胞组和对照组,每组15只.骨髓间充质干细胞组于冠状动脉结扎后1 h在梗死周边区用微量注射器分4点注射自体骨髓间充质干细胞.对照组于相同部位注射等量生理盐水.每点注射体积30 μL.分别于移植后10 min,3 d,4周时各组各处死5只动物.取心脏行冰冻切片,荧光显微镜观察DAPI标记骨髓间充质干细胞的分布情况;免疫荧光法检测标记细胞是否表达心肌特异性肌钙蛋白Ⅰ和α-肌动蛋白.结果与结论:DAPI标记的骨髓间充质干细胞在兔急性心肌梗死模型心脏微环境中,蓝色细胞核分布广泛,呈椭圆形,似心肌细胞核,其排列方向与宿主心肌纤维方向一致,间接免疫荧光法检测胞浆心肌特异性蛋白肌钙蛋白Ⅰ、α-肌动蛋白均为阳性.说明缺血心肌内移植入骨髓间充质干细胞,可在局部微环境的刺激下向心肌细胞定向分化.
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| 关 键 词： | 心肌梗死 骨髓间充质干细胞 细胞移植 肌钙蛋白Ⅰ α-肌动蛋白 |
Differentiation and survival of autologous bone marrow mesenchymal stem cells following transplantation into the myocardium |
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| Cai Hong-yan,Nie Jun,Chen Li-xing,Zhao Ling,Guo Tao,Xiao Jian-ming.Differentiation and survival of autologous bone marrow mesenchymal stem cells following transplantation into the myocardium[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(6). |
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| Authors: | Cai Hong-yan Nie Jun Chen Li-xing Zhao Ling Guo Tao Xiao Jian-ming |
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| Institution: | Cai Hong-yan,Nie Jun,Chen Li-xing,Zhao Ling,Guo Tao,Xiao Jian-ming Department of Cardiology,First Affliated Hospital of Kunming Medical College,Kunming 650031,Yunnan Province,China |
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| Abstract: | BACKGROUND:It was uncertain that the migration,differentiation and survival of transplanted bone marrow mesenchymal stem cells (BMSCs) into myocardium after the acute myocardial infarction.OBJECTIVE:To investigate the migration,differentiation and survival of rabbit transplanted autologous BMSCs in myocardium after the acute myocardial infarction.METHODS:Rabbit BMSCs were isolated and labeled by DAPI in vitro.Rabbit left anterior descending branch was ligated to establish acute myocardial infarction models.... |
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