血浆制备温度和时间对冷沉淀质量的影响

目的 探讨不同血浆制备温度和时间对冷沉淀质量的影响.方法 将新鲜冰冻血浆分成三个温度组通过虹吸法制备冷沉淀,比较融化时间和冷沉淀中FⅧ活性、Fg的含量.结果 2℃、4℃和6℃的融化时间分别为（96.3±5.9） min、（89.6±4.3） min、（81.2±2.7） min.温度越高,所需的融化时间越短.冷沉淀中FⅧ活性分别为（95.36±4.8）IU、（91.21±3.3）IU、（89.8±3.1）IU;冰冻血浆在2℃条件下融化所得冷沉淀中的FⅧ活性最高,与其他两组比较差异具有统计学意义（t=6.94,P=0.009,t =8.96,P=0.004）,但4℃和6℃条件下融化所得冷沉淀中FⅧ活性差异无统计学意义（t=0.669,P=0.19）.冷沉淀中Fg含量分别为（258.6±12.6）mg、（253.3±8.2）mg、（255.9±9.7） mg;不同血浆融化温度对Fg的含量没有显著影响,三组间两两比较差异无统计学意义（t=0.321,P=0.37;t =0.270,P=0.56;=0.424,P=0.29）.结论 2℃条件下血浆融化所制备的冷沉淀中FⅧ活性最高,但是所需时间也最长;6℃条件下融化血浆所制备的冷沉淀FⅧ活性较低,但是符合质量要求,而且所需时间最短,冷沉淀中Fg含量三个温度组之间没有区别.血站可以采用6 ℃融化冰冻新鲜血浆来制备冷沉淀.

Effects of temperature and time on the quality of cryoprecipitate

Objective To investigate the effects of temperature and time on the quality of cryoprecipitate.Methods Fresh frozen plasmas were divided into the three groups,and the cryoprecipitate were prepared from fresh frozen plasma by siphonage method thawing at 2℃,4℃ or 6℃ water bat.Thawing time,FⅧ and fibfinogen （Fg） were compared among the three groups.Results The thawing time at 2℃,4℃ or 6℃ was （96.3 ± 5.9） min,（89.6 ± 4.3） min,（81.2 ± 2.7） min.The activity of F Ⅷ was （95.36 ± 4.8） IU,（91.21 ± 3.3） IU,（89.8 ±3.1）IU,respectively.The activity of FⅧ was the highest at 2℃ condition and there was significantly different compared with other two groups （t =6.94,P =0.009,t =8.96,P =0.004）.The activity of FⅧ in 4℃ group and 6℃ group were comparable （t =0.669,P =0.19）.The concentration of Fg at three groups were similar （258.6 ±12.6） mg,（253.3 ±8.2）mg,（255.9 ±9.7） mg （t =0.321,P =0.37;t =0.270,P =0.56;t =0.424,P =0.29）.Conclusion The activity of FⅧ at 2℃ condition were the highest but the thawing time was the longest.The activity of FⅧ at 6℃ condition were lower but met quality requirements and the thawing time was very short.There was no significantly different at Fg concentration among three groups.Thus 6℃ is more suitable for blood center to prepare cryoprecipitate from fresh frozen plasmas.