| 抑制性消减杂交法分离吗啡依赖大鼠脑内的差异表达基因的初步报道 |
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| 引用本文： | 崔振中,梁德勇,王玢,金蕾,罗非,王晓民,韩济生.抑制性消减杂交法分离吗啡依赖大鼠脑内的差异表达基因的初步报道[J].中国神经科学杂志,2000(1). |
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| 作者姓名： | 崔振中 梁德勇 王玢 金蕾 罗非 王晓民 韩济生 |
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| 作者单位： | 北京医科大学神经科学研究所!北京100083(崔振中，梁德勇，王玢，金蕾，罗非，王晓民)，北京医科大学神经科学研究所!(韩济生) |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 960 0 192 ) |
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| 摘 要： | 用递增剂量的吗啡处理 SD大鼠 ,建立吗啡依赖大鼠模型 ,选取中脑导水管周围灰质、伏核、纹状体等核团 ,提取总 RNA,用抑制性消减杂交 ( SSH)方法 ,分离吗啡依赖大鼠的高表达基因 ,初步获得了 4个新基因的 c DNA片段 ,并发现了 3个已知的功能基因在吗啡依赖过程中增高。进一步的研究尚在进行中。
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| 关 键 词： | 吗啡依赖 抑制性消减杂交 新基因 基因克隆 SD大鼠 |
A preliminary report on the isolation of differentially expressed cDNAs from the brain of morphine dependent rats with SSH |
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| CUI Zhen zhong,LIANG De yong,WANG Bin,JIN Lei,LUO Fei,WANG Xiao min,HAN Ji sheng.A preliminary report on the isolation of differentially expressed cDNAs from the brain of morphine dependent rats with SSH[J].Neuroscience Bulletin,2000(1). |
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| Authors: | CUI Zhen zhong LIANG De yong WANG Bin JIN Lei LUO Fei WANG Xiao min HAN Ji sheng |
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| Abstract: | The programmed increasing doses of morphine were given to SD rats to establish morphine dependent model. Brain areas of periqueductal gray(PAG). nucleus accumbens and striatum were dissected for total RNA extraction. Suppression subtractive hybridization (SSH) method was differentially used for isolating expressed cDNA fragments of morphine dependent rats. Eight highly expressed cDNA fragements were obtained from morphine dependent rat brains, including four new cDNA fragements and 3 known genes. Further studies are still in progress to characterized the full length gene and clarify their functions in morphine dependence. |
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| Keywords: | morphine dependence suppression subtractive hybridization (SSH) new gene gene clone SD rat |
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