| 反相高效液相色谱法测定镇咳宁胶囊中甘草次酸的含量 |
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| 引用本文： | 梁建成,黄义昆,张三平,骆泽宇,蔡震,苏彭美,蓝倩.反相高效液相色谱法测定镇咳宁胶囊中甘草次酸的含量[J].中国药房,2006,17(23):1818-1820. |
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| 作者姓名： | 梁建成 黄义昆 张三平 骆泽宇 蔡震 苏彭美 蓝倩 |
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| 作者单位： | 1. 广西医科大学第五附属医院药剂科,柳州市,545001
2. 桂林医学院,桂林市,541004 |
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| 摘 要： | 目的:采用反相高效液相色谱法测定镇咳宁胶囊中甘草次酸的含量。方法:色谱柱为HypersilC18,流动相为甲醇-水-冰醋酸(87∶13∶2.5),流速为1.0ml/min,检测波长为254nm,柱温为室温。结果:甘草次酸检测浓度在10～120μg/ml范围内线性关系良好(r=0.9999),平均加样回收率为99.68%(RSD=0.49%)。结论:本法简便、快速、准确,可用于镇咳宁胶囊的含量测定。
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| 关 键 词： | 反相高效液相色谱法 镇咳宁胶囊 甘草次酸 含量测定 |
| 文章编号： | 1001-0408(2006)23-1818-03 |
| 收稿时间： | 05 24 2006 12:00AM |
| 修稿时间： | 07 10 2006 12:00AM |
Determination of Glycyrrhetic Acid in Zhenkening Capsules by RP - HPLC |
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| LIANG Jiancheng,HUANG Yikun,ZHANG Sanping,LUO Zeyu,CAI Zhen,SU Pengmei,LAN Qian.Determination of Glycyrrhetic Acid in Zhenkening Capsules by RP - HPLC[J].China Pharmacy,2006,17(23):1818-1820. |
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| Authors: | LIANG Jiancheng HUANG Yikun ZHANG Sanping LUO Zeyu CAI Zhen SU Pengmei LAN Qian |
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| Institution: | 1. Dept. of Pharmacy, The Fifth Affiliated Hospital of Guangxi Medical University, Liuzhou 545001,China;2. Gulin Medical College, Guilin 541004, China |
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| Abstract: | OBJECTIVE:To determine the content of glycyrrhetic acid in zhenkening capsules by RP-HPLC.METHODS:The separation was performed on Hypersil C 18,the mobile phase consisted of methanol-water-glacial acetic acid(87∶13∶2.5)with flow rate at 1.0ml/min and detection wavelength at 254nm,the column temperature was set at room temperature.RE-SULTS:The calibration cure of glycyrrhetic acide was linear in the concentration range of 10～120μg/ml(r=0.9999),the average recovery was 99.68%(RSD=0.49%).CONCLUSION:This method is simple,fast,accurate,and suitable for the determination of glycyrrhetic acid in zhenkening capsules. |
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| Keywords: | RP - HPLC Zhenkening capsule Glyeyrrhetie acid Content determination |
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