| Effects of quercetin on hepatocyte stimulating factor production by mouse peritoneal macrophages |
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| 作者姓名： | 周斌 张俊平 刘宏 殷明 钱定华 |
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| 作者单位： | [1]DepartmentofPharmacology,SchoolofPharmacy [2]DepartmentofStomatology,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai200433,China |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No.39370798,30200344) |
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| 摘 要： | Objective:To study the effects of quercetin on hepatocyte stimulating factor production from mouse peritoneal macrophages.Methods:Hepatocyte stimulating factor was evaluated by the amount of fibrinogen synthesized in Hep3B cvells.Interleukin-6 activity was measured by B9 cell proliferation methyl thiazolyl tetrazolium colorimetric method. Hep3B cell supernatant fibrinogen was quantitated with ELISA.Results:LPS induced the synthesis of hepatocyte stimulating factor in mouse peritoneal macrophages,and hepatocyte stimulating factor promotes the synthesis of fibrinogen from Hep3B cells.Quercetin(5 to 40 μmol/L) inhibited the synthesis of hepatocyte stimulating factor stimulated by LPS.Quercetin(5 to 20 μmol/L) inhibited release of interleukin-6 from mouse peritoneal macrophages induced by 0.5g/L fibrin fibrinogen degradation products.Conclusion:Quercetin inhibits the synthesis of hepatocyte stimulating factor in macrophages.
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| 关 键 词： | 榭皮素 肝细胞刺激性因素 合成纤维蛋白原 白细胞介素－6 巨噬细胞 动物实验 |
Effects of quercetin on hepatocyte stimulating factor production by mouse peritoneal macrophages |
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| ZHOU Bin ZHANG Jun-pin,LIU Hong YIN Ming QIAN Ding-hua.Effects of quercetin on hepatocyte stimulating factor production by mouse peritoneal macrophages[J].Journal of Medical Colleges of PLA(China),2003,18(2):121-123. |
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| Authors: | ZHOU Bin ZHANG Jun-pin LIU Hong YIN Ming QIAN Ding-hua |
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| Institution: | ZHOU Bin ZHANG Jun-pin,LIU Hong YIN Ming QIAN Ding-hua
Department of Pharmacology,School of Pharmacy,Department of Stomatology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | |
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| Keywords: | quercetin hepatocyte stimulating factor fibrinogen interleukin-6 peritoneal macrophage |
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