| BMP-4在视网膜与视神经中的分布及对少突胶质前体细胞分化的影响 |
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| 引用本文： | 刘培培,陈园园,郑华,李艳,余振珏,肖虹蕾,周国民.BMP-4在视网膜与视神经中的分布及对少突胶质前体细胞分化的影响[J].神经解剖学杂志,2010,26(5). |
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| 作者姓名： | 刘培培 陈园园 郑华 李艳 余振珏 肖虹蕾 周国民 |
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| 作者单位： | 复旦大学上海医学院解剖与组织胚胎学系,上海,200032 |
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| 摘 要： | 目的:研究骨形态发生蛋白4(bone morphogenetic protein4,BMP-4)在视网膜与视神经上的表达情况及其对少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)分化的影响,进一步探讨视神经乳头处无髓鞘形成的原因。方法:取生后7d SD大鼠视神经,用免疫组织化学方法研究BMP-4的表达情况。采用恒温振荡法和差速贴壁法分离纯化新生SD大鼠OPCs。用BMP-4诱导OPCs分化,通过免疫细胞化学方法研究OPCs的分化方向。通过Western Blot方法研究BMP-4浓度与OPCs细胞体系内Olig2蛋白表达水平之间的相互关系。结果:(1)在发育过程中,BMP-4仅选择性表达在视神经乳头处;(2)成功纯化的OPCs在PDGF和bFGF撤除后自发分化为少突胶质细胞;(3)OPCs经BMP-4诱导后向II型星形胶质细胞分化;(4)OPCs内Olig2蛋白的表达量随着BMP-4浓度的逐渐增高而逐渐降低。结论:在视神经乳头表达的BMP-4蛋白有可能作用于迁移至此处的OPCs,通过激活相关信号途径以下调OPCs内Olig2蛋白的表达水平,抑制OPCs向少突胶质细胞方向分化,从而抑制视神经乳头髓鞘形成。
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| 关 键 词： | BMP-4 少突胶质前体细胞 Olig2 视神经乳头 无髓鞘形成 |
The distribution of BMP-4 in rat retina and optic nerve and its effects on the differentiation of oligodendrocyte precursors |
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| Liu Peipei,Chen Yuanyuan,Zheng Hua,Li Yan,She Zhenjue,Xiao Honglei,Zhou Guomin.The distribution of BMP-4 in rat retina and optic nerve and its effects on the differentiation of oligodendrocyte precursors[J].Chinese Journal of Neuroanatomy,2010,26(5). |
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| Authors: | Liu Peipei Chen Yuanyuan Zheng Hua Li Yan She Zhenjue Xiao Honglei Zhou Guomin |
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| Institution: | Liu Peipei,Chen Yuanyuan,Zheng Hua,Li Yan,She Zhenjue,Xiao Honglei,Zhou Guomin(Department of Anatomy and Histology and Embryology,Shanghai Medical School of Fudan University,Shanghai 200032,China) |
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| Abstract: | Objective:To research the expression of BMP-4 in retina and optic nerve and its effects to the differentiation of oligodendrocyte precursor cells(OPCs),to further explain the cause of amyelination in optic nerve head.Methods:Frozen sections of longitudinal P7 optic nerve and retina were labeled with antibodies to BMP-4 to define in more details the spatial and temporal distribution of BMP-4.The modified mixed glial culture and different-attachment method were used to harvest OPCs from the P0-P1 postnatal SD... |
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| Keywords: | BMP-4 Olig2 |
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