粒细胞集落刺激因子影响阿糖胞苷诱导HL-60白血病细胞凋亡的研究

目的：观察粒细胞集落刺激因子（G-CSF）对阿糖胞苷（Ara-C）诱导HL-60白血病细胞凋亡的调控作用。方法：应用细胞培养技术、细胞凋亡形态、二苯胺法DNA片段化定量和MTT法来观察HL-60白血病细胞的增殖、凋亡和细胞药敏的变化。结果：G-CSF有刺激HL-60白血病细胞的增殖的作用，其细胞集落为76.5±18.0，而对照组仅为46.5±13.5（P<0.01，n=5）。Ara-C（10 mg/L）作用于HL-60白血病细胞4 h，DNA片段率为32.3%±6.5%，同时加入G-CSF和Ara-C作用于HL-60白血病细胞4 h；DNA片段率为23.6%±7.2%，两组比较有非常显著性差异（P<0.01）。HL-60白血病细胞加入G-CSF后对Ara-C的IC₅₀（18.85±3.21）可看出比单用Ara-C（15.29±2.47）的敏感性低（P<0.05）。结论：G-CSF能刺激HL-60白血病细胞增殖；抑制Ara-C诱导HL-60白血病细胞的凋亡；能降低HL-60白血病细胞对Ara-C的敏感性。

Effect of G-CSF on the apoptosis induced by Ara-c in HL-60 leukemic cells

AIM: To observe the effects of methionine-induced hyperhomocysteinemia on protein C(PC), antithrombin-Ⅲ (AT-Ⅲ) and von willebrand factor (vWF).METHODS:The proliferat ion of HL-60 leukemia cell was observed by hemopoiet ic cell culture.Apoptosis was measured by the morphology of apoptosis cell, the quantitation of DNA fragmentation with the diphenylamine reaction.The change in drug sensitivity was measured by MTT.RESULTS:In group M, the levels of methionine(29.97±5.34 μmol/L) and homocysteine(13.30±2.19 μmol/L) in serum were signifficantly higher than those(14.48±1.97 μmol/L and 5.36±1.19 μmol/L, respectively, P＜0.01) of group C.The levels of AT-Ⅲ and PC of group M were signifficantly lower than those of group C (P＜0.01). The level of vWF in plasma of group M was higher than that of group C (P＜0.01). Immunohistochemistry showed that vWF expression in endothelial cells of aorta was decreased. CONCLUSION:Methionine-induced hyperhomocysteinemia had promotive effects on coagulation and inhibiting effects on antioagulation.