短期给予生长抑素抑制胃癌血管形成的研究

背景与目的:抑制恶性肿瘤的血管形成是目前肿瘤治疗研究的热点之一,本试验旨在探讨皮下注射生长抑素类似物对胃癌血管形成的抑制作用。方法:通过术前胃镜取材及免疫组织化学染色筛选血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)和生长抑素受体2(somatostatinreceptor2,SSTR2)表达阳性的25例胃癌患者纳入本研究。给予善得定10μg/kg皮下注射,每日两次共7天,然后行手术治疗。通过ELISA法检测用药前和用药后第1、3、7天血清VEGF和碱性成纤维细胞生长因子(b-fibroblastgrowthfactor,bFGF)的浓度,应用Westernblot法检测手术前后肿瘤组织VEGF和bFGF的表达。免疫组织化学染色检测手术前后肿瘤微血管密度的变化。结果:用药1周后血清VEGF浓度较用药前显著下降犤(1.02±0.41)μg/Lvs(1.88±0.87)μg/L,P<0.05犦,血清bFGF浓度较用药前显著下降(0.88±0.32)ng/Lvs(2.12±1.06)ng/L,P<0.05;用药后组织VEGF表达显著下降犤(吸光度值:1306vs488,P<0.01)犦,bFGF表达也显著下降(吸光度值:1287vs512,P<0.01)。用药后肿瘤微血管密度有降低趋势,但两者间差异无统计学意义(P>0.05)。结论:短期给予生长抑素可以降低胃癌组织中VEGF和bFGF表达,但对微血管密度的影响无统计学意义。用药后血清VEGF和bFGF浓度随治疗时间

Effects of short-term treatment of somatostatin on angiogenesis of gastric carcinoma

BACKGROUND & OBJECTIVE: It is a hot spot in tumor therapies to inhibit the angiogenesis of carcinoma.Our current study was designed to investigate the inhibitory effect of somatostatin analogue on the angiogenesis of gastric carcinoma by hypodermic injection. METHODS: Twenty-five patients who have positive expression of vascular endothelial growth factor(VEGF) and somatostatin receptor 2(SSTR2)were selected by gastroscopic biopsy and immunohistochemical staining before surgical operation. After 7 days of Sandostatin administration (10 microg/kg, hypodermic injection, twice a day), the tumor extirpations were made. During the Sandostatin treatment, the serum levels of VEGF and the basic fibroblast growth factor(bFGF)were measured by enzyme-linked immunosorbant assay (ELISA) (d1,d3,d7, separately). Before and after operation,the expression levels of VEGF and bFGF were examined by Western blot analysis and the density of micro-vessels was determined by immunohistochemical staining. RESULTS: After a week of Sandostatin therapy,the concentration of VEGF and bFGF in serum was drastically diminished (VEGF:1.02+/-0.41 microg /L vs 1.88+/-0.87 microg/L, P< 0.05;bFGF:0.88+/-0.32 ng/L vs 2.12+/-1.06 ng/L,P< 0.05). The expression levels of VEGF(absorbance:1.306 vs 488,P< 0.01) and bFGF(absorbance:1.287 vs 512,P< 0.01) were obviously decreased after the remedy. The density of micro-vessels turn to the less, but there was no statistical difference before and after operation(P >0.05). CONCLUSION: By the short-term therapy of sandostatin, the expression of VEGF and bFGF can be inhibited. There is a tendency that the serum levels of VEGF and bFGF decrease with the increase of therapy time.