急性脑梗死与6个miniSTR基因座多态性的相关研究

目的探讨急性脑梗死与6个miniSTR基因座的相关性。方法选取急性脑梗死患者100例,正常对照130例,应用荧光标记多重PCR扩增技术扩增6个基因座。310遗传分析仪进行毛细管电泳,用基因收集和分析软件进行数据信息的收集并确定基因型。用直接计数法计算等位基因频率,应用SPSS统计软件对病例组和对照组的各基因座的各个等位基因间进行卡方检验,计算OR值,并对有意义的OR值进行假设检验。结果基因座D10S1248和D22S1045的各个等位基因频率病例组与对照组间均无显著性差异。基因座D14S1434在病例组中的等位基因14、基因座D1S1677的等位基因11、基因座D2S441病例组的等位基因14和基因座D4S2346的等位基因7的基因频率在病例组和对照组间有显著性差异,并且OR值分别为0.433、28.71、2.303和6.828。结论基因座D14S1434的等位基因14可能是急性脑梗死发病的一个保护基因。D1S1677的等位基因11、基因座D2S441的等位基因14和基因座D4S2346的等位基因7可能是急性脑梗死发病的危险基因,与其他基因连锁致病。

The Corteladon between the Acute Cerebral Infarction and Polymorphism of 6 miniSTR

Objective The purpose of this research is to study the correlation between acute cerebral infarction and 6 miniSTR loci.Methods 100 acute cerebral infarction patients and 130 health blood donation volunteers were selected.Fluorescent polymerase chain reaction(PCR)within the one three loci were contained in one tube,then ABI 310 Genetic Analyzer was used to capillary electrophoresis and analyzed the length of allele fragments.Genetic data collection and analysis software was used for data collection and genotyping.Allele frequencies were calculated by direct counting.χ2 test by adopting SPSS10.0 statistical software was used to examine the difference between the cases and controls,The odds ratio was also calculated.Results Each of allele frequences showed no signifi cant difference between cases and controls in D10S1248 and D22S1045 loci.The allele 14 in D14S1434,the allele 11 in D1S1677,the allele 14 in D2S441,and the allele 7 in D4S2346 showed signifi cant difference between cases and controls.The odds ratios were 0.433,28.71,2.303,and 6.828,respectively.Conclusions The allele 14 in D14S1434 may be the protective gene of acute cerebral infarction.3.The allele 11 in D1S1677,the allele 14 in D2S441,and the allele 7 in D4S2346 may be the risk gene of acute cerebral infarction that linked to pathogenesis of this disease.