Phenotypic and functional characterization of a Sézary cell |
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Authors: | Romagnani S. Del Prete G. F. Maggi E. Cadoni A. Corte G. Ghezzi G. Moretti S. Ricci M. |
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Affiliation: | (1) Institute of Clinical Immunology, University of Florence, Florence, Italy;(2) Institute of Anatomy, University of Genoa, Genoa, Italy;(3) Institute of Biochemistry, University of Genoa, Genoa, Italy;(4) Institute of Dermatology, University of Florence, Florence, Italy;(5) Immunologia Clinica Instituto Clinica Medica 3, Policlinico di Careggi, 50134 Firenze, Italy |
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Abstract: | We have studied the surface antigen pattern, enzymatic phenotype, and functional capacity of peripheral blood lymphocytes from a patient with Sézary syndrome (SS). The majority of these cells formed E rosettes but lacked the Fc() receptor. The neoplastic cells were reactive with pan-T cell (OKT3)- and helper T cell (OKT4)-subset monoclonal antibodies; however, they lacked the 5/9 antigen, which identifies a more restricted subset of helper T cells. Most SS cells also reacted with PTF 29.12, a monoclonal antibody which recognizes DR determinants. Only 35% of the cells expressed single, focal accumulations of -naphthyl-acid esterase activity, which is a characteristic of T.M cells, but 85% of them showed this focal staining pattern with acid phosphatase or -glucuronidase. Mononuclear cells from the SS patient showed poor or no proliferative response to phytohemagglutinin, pokeweed mitogen, concanavalin A, purified protein derivative, Candida, and allogeneic cells and lacked both helper and suppressor activity for pokeweed mitogen driven production of IgM and IgG immunoglobulins by normal B cells, but they were able to stimulate a marked proliferative response in mixed-lymphocyte culture. The defective expression of enzymatic and surface membrane characteristics, together with the lack of some T-cell functions, suggests that the patient cells may be immature T.M lymphocytes. |
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Keywords: | Sé zary cell T-cell subpopulations monoclonal antibodies T-cell function assays |
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