微波辐射对大鼠海马神经元损伤作用

目的 观察高功率微波(HPM)辐射后原代培养大鼠海马神经元中钙-钙调蛋白依赖蛋白激酶(Ca²⁺/calmodulin-dependent protein kinaseⅡ,CaMKⅡ)的变化及其意义。方法 采用30 mW/cm²HPM辐射原代培养大鼠海马神经元,于辐射后1 h,采用免疫细胞化学等方法观察海马神经元中磷酸化-CaMKⅡ(p-CaMKⅡ)的变化。结果 30 mW/cm²HMP辐射后1 h,大鼠海马神经元细胞形态有所改变,部分细胞胞体略萎缩,胞浆减少,突起变细,长度变短;免疫细胞化学结果显示,同假辐射组比较,辐射组大鼠海马神经元胞浆中棕黄色颗粒明显增多,颜色加深;免疫荧光结果显示,同假辐射组比较,辐射组大鼠海马神经元胞浆中红色荧光明显增强。p-CaMKⅡ表达明显增加。结论 HPM辐射后大鼠海马原代培养神经元中CaMKⅡ磷酸化增强,参与了海马神经元损伤及突触可塑性改变的病理生理过程。

Injuries of hippocampus neurons of rats after microwave radiation

Objective To investig ate the changes and significance of the expression of CaMK Ⅱ in pr imary cultured hippocampus neurons after high power microwave(HPM)radiation.Methods HPM device was used to radiate primary cultured hippocampus neurons,which were observed 1 h after the radiation.The changes of the expression of p-CaMK Ⅱ in primary cultured hippocampus neurons were analysed by immunohistochemistry and other methods.Results The morphological changes of the neurons were observed within 1 h after 30 mW/cm² radiation.Some neurons were atrophied,the cytoplasm was decreased,the dendrites were shorter and thinner.The levels of p-CaMK Ⅱ in the neur ons were weak at 1 h after 0 mW/cm² radiation and strong at 1 h after the radiation by immunohistochemistry.The levels p-CaMK Ⅱ were increased 1 h after radiation.The levels of p-CaMK Ⅱ in primary culturd hippocampus neurons were also increased 1 h after radiat ion by immunofluorescence and laser scanning confocal microscopy.Conclusion HPM radiation may increase the phosphorylation of CaMK Ⅱ in primary cultured hippocamous neurons,which is contributed to the pathophysiological process of the injuries of hippocampus tissue.