TLR-4介导的免疫逃逸信号转导系统在膀胱尿路上皮癌中的表达及意义

目的：探讨TLR-4介导的免疫逃逸信号转导系统在膀胱尿路上皮癌免疫逃逸 膀胱癌生物学行为的关系。方法：采用免疫组化染色法检测60例膀胱尿路上皮癌和10例正 4、B7-HI、PD-1、Fas及FasL的表达。结果：TLR-4在正常膀胱组织中均有表达，而在膀胱尿路上皮癌中表达有所降低；B7-H1、PD-1、Fas、FasL在正常膀胱组织中无表达或表达极低，而在膀胱尿路上皮癌中的表达均明显增高，并且其表达与肿瘤的病理分级和临床分期密切相关，同时B7-H1表达与复发亦密切相关。结论：TLR-4介导的免疫逃逸信号转导系统可能与膀胱尿路上皮癌的免疫逃逸密切相关，在膀胱尿路上皮癌的发生发展中可能存在着TLR-4介导的免疫逃逸信号转导系统的活化和相关免疫抑制分子表达的上调。

The expression and significance of TLR4-mediated immune escape signal transduction system in bladder urothelial carcinoma

Objective：To investigate the mechanism of the TLR 4-mediated immune escape signal transduction system in bladder urothelial carcinoma and their relationship with the biological behavior of bladder cancer. Meth- od：Sampales from 60 patients with bladder urothelial carcinoma and 10 cases with normal bladder tissue were ex- amined for TLR-4, B7-H1, PD-1, Fas and FasL expression by iramunohistochemical staining Result：TLR-4 posi- tivity was observed in all normal bladder tissue, while decreased expression in bladder urothelial carcinoma; There were no expression or very low expression of B7-H1, PD-1, Fas and FasL in normal bladder tissue, but in bladder urothelial carcinoma the positive expression of those proteins were significantly higher, and those proteins expres- sion were closely correlated with tumor pothological grade and clinical stage, at the same time the B7-H1 expres- sion was also correlated with recurrence. Conclusion： TLR-4-mediated signal transduction system may be associated with bladder urothelial carcinoma immune escape, and activation of TLR-4-mediated immune escape signal trans- duction system may exist in the development of bladder urothelial carcinoma and along with TLR-4-mediated signal transduetion system related immunosuppressive molecule upregulation.