N-乙酰半胱氨酸抑制大鼠肾草酸钙结石形成机制的研究

目的：探讨N一乙酰半胱氨酸（NAc）对大鼠肾草酸钙结石形成的影响及机制，为临床预防尿路结石提供理论依据。方法：将30只健康清洁成年雄性Wistar大鼠先在相同环境下适应性喂养1周，然后随机分为3组：A组（空白对照组）、B组（单纯诱石组）、C组（诱石＋NAC干预组）。A组饮去离子水，B组饮1％乙二醇的去离子水，C组饮1％乙二醇去离子水，并给予NAC100mg／（kg·d）灌胃。第4周处死大鼠，取出双肾，左肾纵向剖开，用10％甲醛固定，HE染色石蜡切片，在100、400倍光镜下观察肾组织草酸钙结晶沉积情况，并进行分级及评分。右肾皮质制成10％的匀浆，检测丙二醛（MDA）及超氧化物歧化酶（SOD）。全部实验数据通过SPSS17．0统计软件分析处理。结果：①肾脏结晶沉积情况分级及评分结果：与B组相比，C组的肾脏结晶沉积评分明显降低（P〈0．05）。②MDA检测结果：与B组相比，C组的MDA含量降低，差异有统计学意义（P〈0．05）。③SOD检测结果：与B组相比，C组的SOD含量增高，差异有统计学意义（P〈0．05）。④A、B、C三组肾组织结晶等级评分与s0D含量的相关系数为-0．499（P〈0．01），结晶评分与MDA含量的相关系数为0．592（P〈0．01）。结论：NAc可以通过其抗氧化作用抑制大鼠肾草酸钙结石的形成。

Research the mechanism of N-acetyl cysteine inhibiting renal calcium oxalate stone formation in rats

Objective：To study the effect and mechanism of N-acetyl cysteine（NAC） on renal calcium oxalate stone formation in rats, For clinical prevention urinary stones provide theoretical basis. Methods： At first, 30 health clean adult male Wistar rats adaptability feeding 1 week in the same environment, then randomly divided into 3 groups（n： 10） ：group A（Blank control group）, group B（stone forming group）, group C（stone forming＋NAC intervention group）, group A drink deionized water, group B drink 1% glycol in deionized water, group C drink 1% glycol in deionized water, and to give NAC100 mg/（kg · d） by lavage. After feeding for 4 weeks, remove double kidney, left renal opened longitudinally,with 10% formaldehyde fixed, HE dyeing paraffin section, the deposit condition of calcium oxalate crystals in nephridial tissue was observed and scored by 100, 400 times light-microscopy. Right renal cortex made 10% of slurry, detection of MDA and SOD. All the experimental data analysis by SPSS17.0 statistical software. Results：①Classification and score the deposit condition of calcium oxalate crystals in nephridial tissue： Compared with group B, group C of kidney crystal deposition score significantly decrease （P〈0.05） ; ②MDA test results： Compared with group B, group C of MDA content decreased, difference have statistical significance（P〈0. 05）; ③SOD test results： Compared with group B, group C of SOD content increased, difference have statistical significance（P〈0.05）; ④The correlation coefficient of score（deposit condition of calcium oxalate crystals in nephridial tissue） and SOD content is -0. 499（P〈0.01）, the correlation coefficient of score and MDA content is 0. 592（P〈0.01）. Conclusions： NAC can inhibiting renal calcium oxalate stone formation in rat through its antioxidation.