B7-H1及其受体PD-1在膀胱癌患者外周血免疫细胞中的表达及其临床意义

目的：研究膀胱癌患者外周血中树突状细胞（dendritic cells，DCs）表面B7-H1和CD8＋T淋巴细胞表面PD-1的表达情况及其临床意义。方法：分离30例膀胱癌患者外周血单个核细胞（peripheral blood mononuclear cell，PBMC），联合培养后采用流式细胞术检测DCs表面B7-HI及CD8＋,T细胞表面PD-1的表达情况。结果：膀胱癌患者外周血DCs表面B7-H1表达水平及CD8＋T细胞表面PD-1表达水平均明显高于正常人，差异具有显著统计学意义（P〈0．01），并且二者的表达水平随着病理分级和临床分期的增加均升高，差异有统计学意义（P〈0．05）。结论：在膀胱癌发生发展过程中存在着DCs表面B7-H1和CD8＋T细胞表面PD-1分子表达的上调，B7-H1／PD-1信号通路可能在T细胞免疫应答的初始阶段亦参与了膀胱癌免疫逃逸的发生。

Expressions and significance of B7-H1 and PD-1 in peripheral blood immune cells from patients with bladder cancer

Objective：To study the expressions and the clinical significance of BT-H1 on dendritic cell and PD-1 on CD8＋T lymphocytes cells in peripheral blood from patients with bladder cancer. Methods： Peripheral blood mononuclear cell were disparted from 30 bladder cancer patients and 10 healthy controls by density gradient centrifugation and then co-cultured. The expressions of BT-H1 on dendritic cells （DCs） and PD-1 on CD8 ＋T lymphocytes were analyzed by flow cytometry. Results： Expressions of BT-H1 on dendritic cells and PD-1 on CD8 ＋T lymphocytes in bladder cancer were higher than healthy controls （P〈0.01）. And the expressions were strongly associated with the pathological grade and clinical stage of bladder cancer （P〈0.05）. Conclusions：The upregulation of BT-H1 on DCs and PD-1 on CD8 ＋T lymphocytes were strongly associated with neoplastic progression of bladder cancer. BT-H1/PD-1 signal passway may also play an important role in immune escape of bladder cancer during initial phase of T cell immune response.