五味子叶化学成分及五味子醇甲对脂多糖诱导的小胶质细胞活化抑制作用的研究

目的 研究北五味子Schisandra chinensis (Turcz.) Baill. 叶的化学成分及化合物五味子醇甲对脂多糖(LPS)诱导的小胶质细胞激活时所分泌的促炎性因子的影响。方法 用色谱法分离并通过波普分析对化合物的结构进行鉴定；采用LPS诱导的小胶质细胞活化模型,以Griess法检测化合物对LPS激活小胶质细胞的抑制作用；以MTT法检测化合物对小胶质细胞细胞成活率的影响。结果 从北五味子叶中分离鉴定了8个化合物：β-谷甾醇(1)、五味子醇乙(2)、五味子乙素(3)、五味子丙素(4)、五味子酯乙(5)、五味子醇甲(6)、(-)-戈米辛M1 (7)、及(-)-戈米辛L1(8)。五味子醇甲可显著抑制激活的N9小胶质细胞的NO释放。结论 首次系统分离并鉴定北五味子叶的化学成分，其中五味子醇甲能明显抑制小胶质细胞的活化。

Studies on chemical constituents of leaf of Schisandra chinensis and inhibitory effect of schizandrin on activation of microglia induced by LPS

Aim To study the chemical constituent of leaf of Schisandra chinensis(Turcz.) Baill.and the effects of schizandrin on lipopolysaccharide(LPS-induced) proinflammatory cytokine production in activated microglia.Methods Eight known compounds were isolated from the leaf of Schisandra chinensis(Turcz.) Baill.By using LPS-induced microglia activation model,drug screening experiments about schizandrin on anti-microglial cell activity were carried out.By MTT,their effects on the survival rate of microglial cells we...