| HPLC/DAD法同时测定小柴胡汤中黄芩苷和甘草酸的含量 |
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| 引用本文： | 未臻宇,刘宇,赵白云,张海,李翔,柴逸峰. HPLC/DAD法同时测定小柴胡汤中黄芩苷和甘草酸的含量[J]. 药学实践杂志, 2006, 24(6): 331-334 |
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| 作者姓名： | 未臻宇 刘宇 赵白云 张海 李翔 柴逸峰 |
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| 作者单位： | 第二军医大学药学院,上海,200433 |
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| 摘 要： | 目的:建立一种反相高效液相色谱分析方法,同时测定小柴胡汤中黄芩苷和甘草酸的含量.方法:采用Agilent Zorbax SB C18柱(4.6mm×250mm,5m),以乙腈-0.01%磷酸溶液为流动相,梯度洗脱,流速1.0mL/min,柱温25℃,检测波长为250nm.结果:黄芩苷和甘草酸单铵盐在50min内即实现良好分离,黄芩苷浓度在12.66～405.0 g/mL线性范围内呈良好的线性关系,其回归方程为:Y=19.726X 13.972,r=0.999 8.甘草酸在1.988～63.60 g/mL的线性范围内呈良好的线性关系,其回归方程为:Y=10.765X 6.643 3,r=0.999 7.两种成分精密度试验RSD＜1%;48h内稳定性试验RSD＜1%;加样回收率试验RSD＜2%.结论:该方法简便快速、准确可靠,能同时测定小柴胡汤中黄芩苷及甘草酸两种有效成分的含量,为有效控制小柴胡汤的质量提供参考.
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| 关 键 词： | 小柴胡汤 黄芩苷 甘草酸 高效液相色谱法 |
| 文章编号： | 1006-0111(2006)06-0331-04 |
| 收稿时间： | 2006-07-14 |
| 修稿时间： | 2006-07-14 |
Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD |
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| ZHU Zhen-yu,LIU Yu,ZHAO Bai-yun,ZHANG Hai,LI Xiang and CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. The Journal of Pharmaceutical Practice, 2006, 24(6): 331-334 |
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| Authors: | ZHU Zhen-yu LIU Yu ZHAO Bai-yun ZHANG Hai LI Xiang CHAI Yi-feng |
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| Affiliation: | School of Pharmacy,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | |
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| Keywords: | Xiaochaihu decoction baicalin glycyrrhizic acid HPLC |
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