β-Glucan attenuates inflammatory responses in oxidized LDL-induced THP-1 cells via the p38 MAPK pathway |
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| Institution: | 1. Chair of Nutritional Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, Warsaw 02-776, Poland;2. Food Biotechnology Department, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wroclaw University of Economics, Komandorska 118/120, Wroclaw 53-345, Poland;3. Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, Warsaw 02-776, Poland;4. Department of Clinical Endoscopy, Institute of Rural Health, Jaczewskiego 2, Lublin 20-090, Poland;1. Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands;2. Laboratory of Food Chemistry, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands;3. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;4. Nuscience Group Headquarters, Booiebos 5, 9031 Ghent (Drongen), Belgium;5. Agrifirm Innovation Center, Landgoedlaan 20, P.O. Box 20018, 7302 HA Apeldoorn, The Netherlands;6. Animal Nutrition Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands;7. Department of Obstetrics and Gynaecology, University of Groningen, and University Medical Center Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands;1. National Research Institute of Chinese Medicine, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan;2. Department of Agronomy, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei 106, Taiwan;3. Graduate Institute of Pharmacognosy, Taipei Medical University, 252 Wu-Hsing St., Taipei 110, Taiwan;4. Institute of Biophotonics, National Yang-Ming University, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan |
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| Abstract: | AimTo investigate the immunomodulatory effects of β-(1,3/1,6)-d-glucan on atherosclerosis as well as on the molecular mechanisms of its transition.Methods and resultsHuman monocytic leukemia (THP-1) cells were differentiated into the macrophage phenotype by incubation with oxLDL in the absence or presence of β-glucan. β-glucan attenuated CD86 and CD80 expression and simultaneously reduced secretion of the inflammatory cytokines IL-2, IL-8, IL-12, TNF-α and IFN-γ. Western blot analysis showed that oxLDL treatment induced phosphorylation of p38 MAPK and ERK1/2 in PMA-differentiated THP-1 cells. However, β-glucan inhibited p38 MAPK activation. In experiments with monocytes derived from healthy donors, β-glucan inhibited IL-8, IL-12 and TNF-α production. The anti-inflammatory effects of β-glucan were also observed in atherosclerotic plaque cells.Conclusionsβ-glucan inhibited oxLDL-induced pro-inflammatory effects in macrophages via regulation of p38 MAPK phosphorylation. This novel finding may provide insight for new therapeutic strategies. |
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| Keywords: | β-Glucan Atherosclerosis p38 MAPK Macrophage |
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