Effect of higher frequency components and duration of vibration on bone
tissue alterations in the rat-tail model |
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Authors: | Srikara V PEELUKHANA Shilpi GOENKA Brian KIM Jay KIM Amit BHATTACHARYA Keith F STRINGER Rupak K BANERJEE |
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Institution: | 1.Department of Mechanical and Materials Engineering, University of Cincinnati, USA;2.Department of Environmental Health, University of Cincinnati, USA;3.Department of Pathology, Cincinnati Children’s Hospital Medical Centre, USA |
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Abstract: | To formulate more accurate guidelines for musculoskeletal disorders (MSD) linked to
Hand-Arm Vibration Syndrome (HAVS), delineation of the response of bone tissue under
different frequencies and duration of vibration needs elucidation. Rat-tails were vibrated
at 125 Hz (9 rats) and 250 Hz (9 rats), at 49 m/s2, for 1D (6 rats), 5D (6
rats) and 20D (6 rats); D=days (4 h/d). Rats in the control group (6 rats for the
vibration groups; 2 each for 1D, 5D, and 20D) were left in their cages, without being
subjected to any vibration. Structural and biochemical damages were quantified using empty
lacunae count and nitrotyrosine signal-intensity, respectively. One-way repeated-measure
mixed-model ANOVA at p<0.05 level of significance was used for
analysis. In the cortical bone, structural damage quantified through empty lacunae count
was significant (p<0.05) at 250 Hz (10.82 ± 0.66) in comparison to the
control group (7.41 ± 0.76). The biochemical damage was significant
(p<0.05) at both the 125 Hz and 250 Hz vibration frequencies. The
structural damage was significant (p<0.05) at 5D for cortical bone
while the trabecular bone showed significant (p<0.05) damage at 20D
time point. Further, the biochemical damage increased with increase in the duration of
vibration with a significant (p<0.05) damage observed at 20D time
point and a near significant change (p=0.08) observed at 5D time point.
Structural and biochemical changes in bone tissue are dependent upon higher vibration
frequencies of 125 Hz, 250 Hz and the duration of vibration (5D, 20D). |
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Keywords: | Hand-arm vibration syndrome Resonance frequency Osteocyte Biochemical alterations Nitrotyrosine |
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