Screening for adenoviruses in haematological neoplasia: High prevalence in mantle cell lymphoma |
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Affiliation: | 1. Children’s Cancer Research Institute, Vienna, Austria;2. Institute of Pathology, Medical University of Vienna, Austria;3. Department of Hematology, Medical University Vienna, Vienna, Austria;4. Department of Oncology, Medical University Vienna, Vienna, Austria;5. Institute of Pathology and Microbiology, Hanusch-Krankenhaus, Vienna, Austria;6. Department of Pediatrics, Medical University Vienna, Vienna, Austria;1. Department of Dermatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands;2. Eindhoven Cancer Registry, Comprehensive Cancer Centre South, Eindhoven, The Netherlands;3. Department of Public Health, Erasmus MC University Medical Center, Rotterdam, The Netherlands;4. Department of Surgical Oncology, Erasmus MC University Medical Center – Daniel den Hoed Cancer Center, Rotterdam, The Netherlands;5. Department of Clinical Epidemiology, Viecuri Medical Centre, Venlo, The Netherlands;1. Institute of Environmental Assessment and Water Research, IDÆA, CSIC, C/Jordi Girona, 18-26, 08034 Barcelona, Spain;2. Institute of Environmental Science and Technology (ICTA), Universitat Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain;1. Indian Institute of Tropical Meteorology, Dr. Homi Bhabha Road, Pune 411008, India;2. Centre of Excellence in Climatology, Birla Institute of Technology, Ranchi 835215, India |
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Abstract: | Human adenoviruses possess oncogenic capacity which is well documented in mammalian animal models, but their possible implication in human malignancy has remained enigmatic. Following primary infection, adenoviruses can persist in a latent state in lymphocytes where the virus is apparently able to evade immune surveillance. In the present study, we have employed a broad-spectrum adenovirus polymerase chain reaction (PCR) assay to systematically screen more than 200 diagnostic specimens of different lymphoid malignancies including acute lymphocytic leukaemia (n = 50), chronic lymphocytic leukaemia (n = 50), various types of malignant lymphoma (n = 100) and multiple myeloma (n = 11) for the presence of adenoviral sequences. While most entities analysed revealed negative findings in virtually all specimens tested, adenoviral DNA was detected in 15/36 (42%) mantle cell lymphomas investigated. The most prevalent adenoviral species detected was C, and less commonly B. Adenovirus-positive findings in patients with mantle cell lymphoma were made at different sites including bone marrow (n = 7), intestine (n = 5), lymph nodes (n = 2) and tonsillar tissue (n = 1). The presence of adenoviral sequences identified by PCR was confirmed in individual cells by fluorescence in-situ hybridisation (FISH). The frequent observation of adenoviruses in mantle cell lymphoma is intriguings, and raises questions about their possible involvement in the pathogenesis of this lymphoid malignancy. |
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Keywords: | Human adenovirus Oncogenic virus Mantle cell lymphoma Leukaemia Lymphoma |
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