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Improved sectioned images and surface models of the whole dog body
Affiliation:1. Department of Anatomy, Keimyung University School of Medicine, 1095 Dalgubeol-daero, Daegu 704-701, Republic of Korea;2. Department of Anatomy, Ajou University School of Medicine, Suwon 443-749, Republic of Korea;3. Department of Emergency Medicine, Dongguk University School of Medicine, Gyeongju 780-350, Republic of Korea;4. Department of Anatomy, Dongguk University School of Medicine, 87 Dongdae-ro, Gyeongju 780-350, Republic of Korea;1. Department of Orthopaedic, Trauma and Reconstructive Surgery, Charité-University of Medicine, Campus Benjamin Franklin, Berlin, Germany;2. Department of Otorhinolaryngology, Head and Neck Surgery, Charité-University of Medicine, Campus Benjamin Franklin, Berlin, Germany;1. Department of Internal Medicine B, University Hospital of Strasbourg (HUS), Strasbourg, France;2. Unit of Human Sounds Analysis and Laboratory of Research in Pedagogy in Human Health, University of Medicine, University of Strasbourg (UdS), Strasbourg, France;3. Independent researcher, Member, Alcatel Lucent Academy, Bolsenheim, France;1. Department of Medicine, University of Washington, Seattle;2. Department of Medicine, University of California San Francisco;3. Department of Medicine, University of Toronto, Ontario, Canada;1. College of Biological Sciences, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China;2. College of Animal Science and Technology, and College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, PR China;3. Research Center of Cattle and Sheep Embryo Engineering Technique of Hebei Province, Baoding 071000, PR China;1. LUNAM Université, Angers, France;2. CHU Angers, Service de Néphrologie-Dialyse-Transplantation, Angers, France;3. CHU Angers, Service de Gastro-enterologie, Angers, France;1. Department of Animal Science, Iowa State University, Ames, IA 50011, USA;2. Formerly: OXEA Corporation, Bishop, TX 78343, USA;3. OXEA GmbH, Otto-Roelen-Strasse 3, 46147 Oberhausen, Germany;4. Laboratory Animal Resources, Iowa State University, Ames, IA 50011, USA
Abstract:The objective of this research was to produce high-quality sectioned images of a whole dog which can be used to create sectional anatomy atlases and three-dimensional (3D) models. A year old female beagle was sacrificed by potassium chloride injection and frozen. The frozen dog was then serially ground using a cryomacrotome. Sectioned surfaces were photographed using a digital camera to create 3555 sectioned images of whole dog body (intervals, 0.2 mm; pixel size, 0.1 mm; 48 bit color). In a sectioned image, structures of dimension greater than 0.1 mm could be identified in detail. Photoshop was used to make segmented images of 16 structures. Sectioned and segmented images were stored in browsing software to allow easy access. Segmented images were reconstructed to make surface models of 16 structures using Mimics software and stored in portable document format (PDF) using Adobe 3D Reviewer software. In this research, state-of-art sectioned images and surface models were produced for the dog. The authors hope that the sectioned images produced will become a useful source of software for basic and clinical veterinary medicine, and therefore, are distributing the sectioned images and surface models through browsing software and PDF file available free of charge.
Keywords:Dogs  Whole body imaging  Cross sectional anatomy  Magnetic resonance imaging  Three-dimensional imaging  Visible human project
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